Induction of metallothionein in human skin by routine exposure to sunlight: Evidence for a systemic response and enhanced induction at certain body sites

Ablett, E, Whiteman, DC, Boyle, GM, Green, AC and Parsons, PG (2003) Induction of metallothionein in human skin by routine exposure to sunlight: Evidence for a systemic response and enhanced induction at certain body sites. Journal of Investigative Dermatology, 120 2: 318-324. doi:10.1046/j.1523-1747.2003.12025.x


Author Ablett, E
Whiteman, DC
Boyle, GM
Green, AC
Parsons, PG
Title Induction of metallothionein in human skin by routine exposure to sunlight: Evidence for a systemic response and enhanced induction at certain body sites
Journal name Journal of Investigative Dermatology   Check publisher's open access policy
ISSN 0022-202X
Publication date 2003
Sub-type Article (original research)
DOI 10.1046/j.1523-1747.2003.12025.x
Volume 120
Issue 2
Start page 318
End page 324
Total pages 7
Editor D. Norris
Place of publication Malden, U.S.A.
Publisher Blackwell Publishing
Collection year 2003
Language eng
Subject C1
321015 Oncology and Carcinogenesis
730117 Skin and related disorders
Abstract Expression of metallothionein, an antioxidant induced by a variety of stimuli including ultraviolet light, was quantitated by immunohistochemistry in the skin of males aged over 50 who had known short- and long-term exposures to sunlight. Skin punch biopsies were taken from two sites in each subject: the hand in all subjects and a range of other sites matched to patients with a previously excised primary melanoma. Metallothionein expression (strongest in the basal layers of the epidermis and primarily nuclear) was associated with both short- and long-term exposure to sunlight. A plateau of staining intensity was reached after 3 h sun exposure, within the previous 3 d before biopsy. Expression was also elevated in the nonexposed skin sites of subjects who had recent sun exposure, indicating a systemic response to exposure of remote sites. Using the skin of the hand to normalize responses to chronic exposure between individuals, the systemically modulated response to sunlight was significantly greater on the unexposed back than on other sites. The possibility of ultraviolet-induced cytokines selectively modifying the response of skin on a site-specific basis was investigated. The circulating leukocytes, but not lymphocytes, of two individuals exposed to 1 minimal erythema dose whole-body solar-simulated ultraviolet showed increased interleukin-6 mRNA 4 h after exposure. Interleukin-6 was not directly induced in these cell populations 4 h after ultraviolet A or ultraviolet B irradiation ex vivo . Leukocytes may therefore contribute to and amplify the systemic effects of ultraviolet-induced interleukin-6 and metallothionein expression.
Keyword Dermatology
Image Analysis
Immunohistochemistry
Metallothionein
Molecular Epidemiology
Ultraviolet
Mouse Skin
Ultraviolet-radiation
Cutaneous Melanoma
Oxidative Stress
Gene-expression
B Radiation
Cancer
P53
Irradiation
Queensland
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2004 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Tue, 14 Aug 2007, 19:38:29 EST