The blood volumes of the primary and secondary circulatory system in the Atlantic cod Gadus morhua L., using plasma bound Evans Blue and compartmental analysis

Skov, P. V. and Steffensen, J. F. (2003) The blood volumes of the primary and secondary circulatory system in the Atlantic cod Gadus morhua L., using plasma bound Evans Blue and compartmental analysis. Journal of Experimental Biology, 206 3: 591-599. doi:10.1242/jeb.00113

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Author Skov, P. V.
Steffensen, J. F.
Title The blood volumes of the primary and secondary circulatory system in the Atlantic cod Gadus morhua L., using plasma bound Evans Blue and compartmental analysis
Journal name Journal of Experimental Biology   Check publisher's open access policy
ISSN 0022-0949
Publication date 2003-02-01
Sub-type Article (original research)
DOI 10.1242/jeb.00113
Open Access Status File (Publisher version)
Volume 206
Issue 3
Start page 591
End page 599
Total pages 9
Place of publication United Kingdom
Publisher The Company of Biologists Ltd
Collection year 2003
Language eng
Subject C1
321017 Orthopaedics
780105 Biological sciences
0699 Other Biological Sciences
Abstract The volume of the primary (PCS) and secondary (SCS) circulatory system in the Atlantic cod Gadus morhua was determined using a modified dye dilution technique. Cod (N=10) were chronically cannulated in the second afferent branchial artery with PE-50 tubing. Evans Blue dye was bound to harvested fish plasma at a concentration of 1 mg dye ml(-1) plasma, and injected at a concentration of 1 mg kg(-1) body mass. Serial sampling from the cannula produced a dye dilution curve, which could be described by a double exponential decay equation. Curve analysis enabled the calculation of the primary circulatory and total distribution volume. The difference between these volumes is assumed to be the volume of the SCS. From the dilution curve, it was also possible to calculate flow rates between and within the systems. The results of these experiments suggest a plasma volume in the PCS of 3.42+/-0.89 ml 100 g(-1) body mass, and in the SCS of 1.68+/-0.35 ml 100 g(-1) body mass (mean +/- S.D.) or approximately 50% that of the PCS. Flow rates to the SCS were calculated as 2.7% of the resting cardiac output. There was an allometric relationship between body mass and blood volumes. Increasing condition factor showed a tendency towards smaller blood volumes of the PCS, expressed as percentage body mass, but this was not evident for the volume of the SCS.
Keyword Secondary Circulation
Volume
Flow
Leak
Evans Blue
Plasma-bound Tracer
Atlantic Cod
Biology
Gadus Morhua
Trout Salmo-gairdneri
Rainbow-trout
Vessel System
Whole-body
Albumin
Teleost
Cell
Fish
Permeability
Tissue
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2004 Higher Education Research Data Collection
School of Biological Sciences Publications
 
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