Analytical exclusion chromatography

Winzor, D. J. (2003) Analytical exclusion chromatography. Journal of Biochemical And Biophysical Methods, 56 1-3: 15-52.


Author Winzor, D. J.
Title Analytical exclusion chromatography
Journal name Journal of Biochemical And Biophysical Methods   Check publisher's open access policy
ISSN 0165-022X
Publication date 2003
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/S0165-022X(03)00071-X
Volume 56
Issue 1-3
Start page 15
End page 52
Total pages 38
Place of publication The Netherlands
Publisher Elsevier
Collection year 2003
Language eng
Subject C1
270101 Analytical Biochemistry
780105 Biological sciences
Abstract This review summarizes the development of exclusion chromatography, also termed gel filtration, molecular-sieve chromatography and gel permeation chromatography, for the quantitative characterization of solutes and solute interactions. As well as affording a means of determining molecular mass and molecular mass distribution, the technique offers a convenient way of characterizing solute selfassociation and solute-ligand interactions in terms of reaction stoichiometry and equilibrium constant. The availability of molecular-sieve media with different selective porosities ensures that very little restriction is imposed on the size of solute amenable to study. Furthermore, access to a diverse array of assay procedures for monitoring the column eluate endows analytical exclusion chromatography with far greater flexibility than other techniques from the viewpoint of solute concentration range that can be examined. In addition to its widely recognized prowess as a means of solute separation and purification, exclusion chromatography thus also possesses considerable potential for investigating the functional roles of the purified solutes. (C) 2003 Elsevier Science B.V. All rights reserved.
Keyword Biochemical Research Methods
Biochemistry & Molecular Biology
Biophysics
Exclusion Chromatography
Ligand Binding
Molecular Mass Estimation
Solute Self-association
Concentration-dependent Migration
Molecular-sieve Chromatography
Gel-permeation Chromatography
Interacting Protein Systems
Reversibly Polymerizing Solutes
Analytical-band Centrifugation
Muscle Lactate-dehydrogenase
Chemically Reacting Systems
Equilibrium-constants
Self-association
Q-Index Code C1

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Excellence in Research Australia (ERA) - Collection
2004 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Tue, 14 Aug 2007, 19:35:55 EST