Nerve growth factor overexpression and autocrine loop in breast cancer cells

Dolle, Laurent, El Yazidi-Belkoura, Ikram, Adriaenssens, Eric, Nurcombe, Victor and Hondermarck, Hubert (2003) Nerve growth factor overexpression and autocrine loop in breast cancer cells. Oncogene, 22 36: 5592-5601. doi:10.1038/sj.onc.1206805


Author Dolle, Laurent
El Yazidi-Belkoura, Ikram
Adriaenssens, Eric
Nurcombe, Victor
Hondermarck, Hubert
Title Nerve growth factor overexpression and autocrine loop in breast cancer cells
Journal name Oncogene   Check publisher's open access policy
ISSN 0950-9232
Publication date 2003-08-28
Sub-type Article (original research)
DOI 10.1038/sj.onc.1206805
Volume 22
Issue 36
Start page 5592
End page 5601
Total pages 10
Place of publication London, England
Publisher Nature Publishing Group
Collection year 2003
Language eng
Subject C1
321015 Oncology and Carcinogenesis
730108 Cancer and related disorders
Abstract We show here that nerve growth factor (NGF), the canonical neurotrophic factor, is synthesized and released by breast cancer cells. High levels of NGF transcript and protein were detected in breast cancer cells by reverse transcription-PCR, Western blotting, ELISA assay and immunohistochemistry. Conversely, NGF production could not be detected in normal breast epithelial cells at either the transcriptional or protein level. Confocal analysis indicated the presence of NGF within classical secretion vesicles. Breast cancer cell-produced NGF was biologically active, as demonstrated by its ability to induce the neuronal differentiation of embryonic neural precursor cells. Importantly, the constitutive growth of breast cancer cells was strongly inhibited by either NGF-neutralizing antibodies or K-252a, a pharmacological inhibitor of NGF receptor TrkA, indicating the existence of an NGF autocrine loop. Together, our data demonstrate the physiological relevance of NGF in breast cancer and its potential interest as a marker and therapeutic target.
Keyword Breast Cancer
Nerve Growth Factor
Autocrine Stimulation
Cellular Growth
Biochemistry & Molecular Biology
Oncology
Cell Biology
Genetics & Heredity
Stromal Cells
Receptors
Expression
Proliferation
Neurotrophins
Survival
Release
Q-Index Code C1

 
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Created: Tue, 14 Aug 2007, 19:33:40 EST