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Amino acids 1-20 of the hepatitis C virus (HCV) core protein specifically inhibit HCV IRES- dependent translation in Hep G2 cells, and inhibit both HCV IRES- and cap-dependent translation in HuH7 and CV-1 cells.
Li, Dongsheng, Takyar, Seyed Taghi, Lott, William B. and Gowans, Eric J. (2003) Amino acids 1-20 of the hepatitis C virus (HCV) core protein specifically inhibit HCV IRES- dependent translation in Hep G2 cells, and inhibit both HCV IRES- and cap-dependent translation in HuH7 and CV-1 cells.. Journal of General Virology, 84 4: 815-825.
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| Author(s) |
Li, Dongsheng
Takyar, Seyed Taghi
Lott, William B.
Gowans, Eric J.
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| Title |
Amino acids 1-20 of the hepatitis C virus (HCV) core protein specifically inhibit HCV IRES- dependent translation in Hep G2 cells, and inhibit both HCV IRES- and cap-dependent translation in HuH7 and CV-1 cells.
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| Journal name |
Journal of General Virology
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| Publication date |
2003
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| Volume number |
84
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| Issue number |
4
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| ISSN |
0022-1317
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| Start page |
815
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| End page |
825
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| Total pages |
11
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| Editor(s) |
G. L. Smith
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| Place of publication |
Great Britain
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| Publisher |
Society for General Microbiology
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| Collection year |
2003
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| Language |
eng
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| Subject |
C1
270303 Virology
730101 Infectious diseases
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| Abstract |
A self-modulating mechanism by the hepatitis C virus (HCV) core protein has been suggested to influence the level of HCV replication, but current data on this subject are contradictory. We examined the effect of wild-type and mutated core protein on HCV IRES- and cap-dependent translation. The wild-type core protein was shown to inhibit both IRES- and cap-dependent translation in an in vitro system. This effect was duplicated in a dose-dependent manner with a synthetic peptide representing amino acids 1-20 of the HCV core protein. This peptide was able to bind to the HCV IRES as shown by a mobility shift assay. In contrast, a peptide derived from the hepatitis B virus (HBV) core protein that contained a similar proportion of basic residues was unable to inhibit translation or bind the HCV IRES. A recombinant vaccinia-HCV core virus was used to examine the effect of the HCV core protein on HCV IRES-dependent translation in cells and this was compared with the effects of an HBV core-recombinant vaccinia virus. In CV-1 and HuH7 cells, the HCV core protein inhibited translation directed by the IRES elements of HCV, encephalomyocarditis virus and classical swine fever virus as well as cap-dependent translation, whereas in HepG2 cells, only HCV IRES-dependent translation was affected. Thus, the ability of the HCV core protein to selectively inhibit HCV IRES-dependent translation is cell-specific. N-terminal truncated (aa 1-20) HCV core protein that was expressed from a novel recombinant vaccinia virus in cells abrogated the inhibitory phenotype of the core protein in vivo, consistent with the above in vitro data.
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| Keyword(s) |
Hepatitis C virus
HCV
amino acids 1-20
IRES-dependent translation
HepG2 cells
HuH7 cells
CV-1 cells
RNA Replication
Virology
Ribosome Entry Site
Hypervariable Region-1
Structural Proteins
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