Kunjin virus replicon vectors for human immunodeficiency virus vaccine development

Tracey Harvey, Anraku, Itaru, Linedale, Richard, Harrich, David, Mackenzie, Jason, Suhrbier, Andreas and Khromykh, Alexander A. (2003) Kunjin virus replicon vectors for human immunodeficiency virus vaccine development. Journal of Virology, 77 14: 7796-7803. doi:10.1128/JVI.77.14.7796-7803.2003

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Author Tracey Harvey
Anraku, Itaru
Linedale, Richard
Harrich, David
Mackenzie, Jason
Suhrbier, Andreas
Khromykh, Alexander A.
Title Kunjin virus replicon vectors for human immunodeficiency virus vaccine development
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
Publication date 2003-07
Year available 2003
Sub-type Article (original research)
DOI 10.1128/JVI.77.14.7796-7803.2003
Open Access Status File (Publisher version)
Volume 77
Issue 14
Start page 7796
End page 7803
Total pages 8
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2003
Language eng
Subject C1
270303 Virology
730101 Infectious diseases
Abstract We have previously demonstrated the ability of the vaccine vectors based on replicon RNA of the Australian flavivirus Kunjin (KUN) to induce protective antiviral and anticancer CD8(+) T-cell responses using murine polyepitope as a model immunogen (I. Anraku, T. J. Harvey, R. Linedale, J. Gardner, D. Harrich, A. Suhrbier, and A. A. Khromykh, J. Virol. 76:3791-3799, 2002). Here we showed that immunization of BALB/c mice with KUN replicons encoding HIV-1 Gag antigen resulted in induction of both Gag-specific antibody and protective Gag-specific CD8(+) T-cell responses. Two immunizations with KUNgag replicons in the form of virus-like particles (VLPs) induced anti-Gag antibodies with titers of greater than or equal to1:10,000. Immunization with KUNgag replicons delivered as plasmid DNA, naked RNA, or VLPs induced potent Gag-specific CD8(+) T-cell responses, with one immunization of KUNgag VLPs inducing 4.5-fold-more CD8(+) T cells than the number induced after immunization with recombinant vaccinia virus carrying the gag gene (rVVgag). Two immunizations with KUNgag VLPs also provided significant protection against challenge with rVVgag. Importantly, KUN replicon VLP vaccinations induced long-lasting immune responses with CD8(+) T cells able to secrete gamma interferon and to mediate protection 6 to 10 months after immunization. These results illustrate the potential value of the KUN replicon vectors for human immunodeficiency virus vaccine design.
Keyword Vaccine Vectors
Hiv Vaccine
Cellular Immune-responses
Cytotoxic T-lymphocytes
Type-1 Gag
Hiv-1 Infection
Heterologous Genes
Q-Index Code C1
Institutional Status UQ

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Created: Tue, 14 Aug 2007, 19:14:32 EST