Immunohistochemical localization of fibromodulin in the periodontium during cementogenesis and root formation in the rat molar

Matias, M.A., Li, H., Young, W.G. and Bartold, P.M. (2003) Immunohistochemical localization of fibromodulin in the periodontium during cementogenesis and root formation in the rat molar. Journal of Periodontal Research, 38 5: 502-507. doi:10.1034/j.1600-0765.2003.00682.x


Author Matias, M.A.
Li, H.
Young, W.G.
Bartold, P.M.
Title Immunohistochemical localization of fibromodulin in the periodontium during cementogenesis and root formation in the rat molar
Journal name Journal of Periodontal Research   Check publisher's open access policy
ISSN 0022-3484
1600-0765
Publication date 2003-10
Sub-type Article (original research)
DOI 10.1034/j.1600-0765.2003.00682.x
Volume 38
Issue 5
Start page 502
End page 507
Total pages 6
Editor R.C. Page
Place of publication Copenhagen, Denmark
Publisher Blackwell Munksgaard
Collection year 2003
Language eng
Subject C1
320899 Dentistry not elsewhere classified
730112 Oro-dental and disorders
Abstract Background: Cementum is essential for periodontal regeneration, as it provides anchorage between the root surface and the periodontal ligament. A variety of macromolecules present in the extracellular matrix of the periodontium, including proteoglycans, are likely to play a regulatory role in cementogenesis. Recently, the small leucine-rich proteoglycan, fibromodulin, has been isolated from bovine periodontal ligament and localized in bovine cementum, as well as in human periodontal ligament. Objective: The aim of this study was to examine the distribution of fibromodulin during cementogenesis and root formation. Methods: A standard indirect immunoperoxidase technique was employed, using an antifibromodulin polyclonal antibody on sections of molar teeth from rats aged 3, 5 and 8 weeks. Results: Immunoreactivity to fibromodulin was evident in the periodontal ligament in all sections. An intense positive stain was observed in the extracellular matrix where the periodontal ligament fibers insert into the alveolar bone and where the Sharpey's fibers insert into the cementum. There was no staining evident in the mineralized cellular and acellular cementum. The intensity of immunoreactivity to the antifibromodulin antibody increased proportionally with increasing tissue maturation. Conclusion: The results from this study suggest that fibromodulin is a significant component of the extracellular matrix in the periodontal ligament during development, and may play a regulatory role in the mineralization process or maintaining homeostasis at the hard-soft tissue interface during cementogenesis.
Keyword Dentistry, Oral Surgery & Medicine
Fibromodulin
Proteoglycan
Cementum
Periodontal Ligament
Collagen Fibrillogenesis
Differential Expression
Human Cementum
Proteoglycans
Lumican
Glycosaminoglycans
Identification
Inhibition
Healthy
Tissues
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2004 Higher Education Research Data Collection
School of Dentistry Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 23 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 14 Aug 2007, 19:08:18 EST