A synthetic xylanase as a novel reporter in plants

Vickers, C. E., Xue, G. P. and Gresshoff, P. M. (2003) A synthetic xylanase as a novel reporter in plants. Plant Cell Reports, 22 2: 135-140.


Author Vickers, C. E.
Xue, G. P.
Gresshoff, P. M.
Title A synthetic xylanase as a novel reporter in plants
Journal name Plant Cell Reports   Check publisher's open access policy
ISSN 0721-7714
Publication date 2003
Sub-type Article (original research)
DOI 10.1007/s00299-003-0667-9
Volume 22
Issue 2
Start page 135
End page 140
Total pages 6
Place of publication New York, USA
Publisher Springer-Verlag
Collection year 2003
Language eng
Subject C1
270201 Gene Expression
620102 Barley
Abstract Transient gene expression assays are often used to screen promoters before stable transformation. Current transient quantification methods have several problems, including a lack of reporter gene stability and expense. Here we report a synthetic, codon-optimised xylanase gene (sXynA) as a reporter gene for quantitative transient analyses in plants. Azurine-crosslinked xylan (AZCL-xylan) was used as a substrate for assaying xylanase activity. The enzymatic nature of the protein allows for sensitive assays at the low levels of transgene protein found in transiently transformed tissue extracts. The xylanase (XYN) protein is stable, activity slopes are linear over long time periods and assays are cost-effective. Coupled with the GUSPlus reporter gene, the XYN reporter allows sensitive and accurate quantification of gene control sequences in transient expression systems.
Keyword Plant Sciences
Promoter Screening
Reporter Gene
Transient Expression Analysis
Gusplus
Xylanase
High-level Expression
Ubiquitin Promoter
Transgenic Rice
Gene-expression
Maize
Marker
Tobacco
Binding
Barley
Cells
Q-Index Code C1

 
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Created: Tue, 14 Aug 2007, 19:05:32 EST