Decreased expression of the Id3 gene at 1p36.1 in ovarian adenocarcinomas

Arnold, J. M., Mok, S. C., Purdie, D. and Chenevix-Trench, G. (2001) Decreased expression of the Id3 gene at 1p36.1 in ovarian adenocarcinomas. British Journal of Cancer, 84 3: 352-359. doi:10.1054/bjoc.2000.1620

Author Arnold, J. M.
Mok, S. C.
Purdie, D.
Chenevix-Trench, G.
Title Decreased expression of the Id3 gene at 1p36.1 in ovarian adenocarcinomas
Journal name British Journal of Cancer   Check publisher's open access policy
ISSN 0007-0920
Publication date 2001
Sub-type Article (original research)
DOI 10.1054/bjoc.2000.1620
Open Access Status DOI
Volume 84
Issue 3
Start page 352
End page 359
Total pages 8
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2002
Language eng
Abstract The molecular events that drive the initiation and progression of ovarian adenocarcinoma are not well defined. We have investigated changes in gene expression in ovarian cancer cell lines compared to an immortalized human ovarian surface epithelial cell line (HOSE) using a cDNA array. We identified 17 genes that were under-expressed and 10 genes that were over-expressed in the cell lines compared to the HOSE cells. One of the genes under-expressed in the ovarian cancer cell lines, Id3, a transcriptional inactivator, was selected for further investigation. Id3 mRNA was expressed at reduced levels in 6 out of 9 ovarian cancer cell lines compared to the HOSE cells while at the protein level, all 7 ovarian cancer cell lines examined expressed the Id3 protein at greatly reduced levels. Expression of Id3 mRNA was also examined in primary ovarian tumours and was found in only 12/38 (32%) cases. A search was conducted far mutations of Id3 in primary ovarian cancers using single stranded conformation polymorphism (SSCP) analysis. Only one nucleotide substitution, present also in the corresponding constitutional DNA, was found in 94 ovarian tumours. Furthermore no association was found between LOH at 1p36 and lack of expression of Id3. These data suggest that Id3 is not the target of LOH at 1p36. (C) 2001 Cancer Research Campaign.
Keyword Oncology
Ovarian Cancer
Tumour Suppressor Gene
Cdna Array
Loss Of Heterozygosity
Candidate Tumor-suppressor
Surface Epithelium
Tyrosine Kinase
Frequent Loss
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Public Health Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 24 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 29 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 19:04:51 EST