Mechanisms for the inhibition of amiloride-sensitive Na+ absorption by extracellular nucleotides in mouse trachea

Kunzelmann, Karl, Schreiber, Rainer and Cook, David (2002) Mechanisms for the inhibition of amiloride-sensitive Na+ absorption by extracellular nucleotides in mouse trachea. Pflugers Archiv-european Journal of Physiology, 444 1-2: 220-226. doi:10.1007/s00424-002-0796-y


Author Kunzelmann, Karl
Schreiber, Rainer
Cook, David
Title Mechanisms for the inhibition of amiloride-sensitive Na+ absorption by extracellular nucleotides in mouse trachea
Journal name Pflugers Archiv-european Journal of Physiology   Check publisher's open access policy
ISSN 0031-6768
Publication date 2002
Sub-type Article (original research)
DOI 10.1007/s00424-002-0796-y
Volume 444
Issue 1-2
Start page 220
End page 226
Total pages 7
Editor K. Thurau
Place of publication Berlin, Germany
Publisher Springer-Verlag
Collection year 2002
Language eng
Subject C1
270104 Membrane Biology
780105 Biological sciences
Abstract Purinergic stimulation of airway epithelial cells induces Cl- secretion and modulates Na+ absorption by an unknown mechanism. To gain insight into this mechanism, we used a perfused micro-Ussing chamber to assess transepithelial voltage (V-te) and amiloride-sensitive short-circuit current (Isc-Amil) in mouse trachea. Exposure to apical ATP or UTP (each 100 mumol/l) caused a large initial increase in lumen negative V-te and I-sc corresponding to a transient Cl- secretion, while basolateral application of ATP/UTP induced only a small secretory response. Luminal, but not basolateral, application of nucleotides was followed by a sustained and reversible inhibition of Isc-Amil that was independent of extracellular Ca2+ or activation of protein kinase C and was not induced by carbachol (100 mumol/l) or the Ca2+ ionophore ionomycin (1 mumol/l). Removal of extracellular Cl- or exposure to 200 muM DIDS reduced UTP-mediated inhibition of Isc-Amil Substantially. The phospholipase inhibitor U73122 (10 mumol/l) and pertussis toxin (PTX 200 ng/ml) both attenuated UTP-induced Cl- secretion and inhibition of Isc-Amil. Taken together, these data imply a contribution of Cl- conductance and PTX-sensitive G proteins to nucleotide-dependent inhibition of the amiloride-sensitive Na+ current in the mouse trachea.
Keyword Physiology
Purinergic Receptors
Amiloride-sensitive Na+ Channels
Enac
I-sc
Short-circuit Current
Pertussis Toxin
Cystic Fibrosis
Cftr
Trachea
Airways
Mouse Colon
Cl- Secretion
Epithelial Transport
Ussing Chamber
Dids
Utp
Atp
Transmembrane Conductance Regulator
Nucleoside Diphosphate Kinase
Airway Epithelial-cells
Cystic-fibrosis Airways
Ion-transport
Xenopus Oocytes
G-protein
Uridine 5'-triphosphate
Cl Transport
Wild-type
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Tue, 14 Aug 2007, 19:00:42 EST