Macropodid herpesvirus 1 encodes genes for both thymidylate synthase and ICP34.5

Guliani, Sandeep, Polkinghorne, Ian, Smith, Greg A., Young, Peter, Mattick, John S. and Mahony, Timothy J. (2002) Macropodid herpesvirus 1 encodes genes for both thymidylate synthase and ICP34.5. Virus Genes, 24 3: 207-213. doi:10.1023/A:1015396430131


Author Guliani, Sandeep
Polkinghorne, Ian
Smith, Greg A.
Young, Peter
Mattick, John S.
Mahony, Timothy J.
Title Macropodid herpesvirus 1 encodes genes for both thymidylate synthase and ICP34.5
Journal name Virus Genes   Check publisher's open access policy
ISSN 0920-8569
Publication date 2002-06-01
Sub-type Article (original research)
DOI 10.1023/A:1015396430131
Volume 24
Issue 3
Start page 207
End page 213
Total pages 7
Place of publication Netherlands
Publisher Kluwer Academic Publications
Collection year 2002
Language eng
Subject C1
270205 Genetic Development (incl. Sex Determination)
780105 Biological sciences
Abstract Macropodid herpesvirus 1 (MaHV-1) is an unclassified alphaherpesvirus linked with the fatal infections of kangaroos and other marsupials. During the characterisation of the internal repeat region of MaHV-1, an open reading frame (ORF) encoding for thymidylate synthase (TS) gene was identified and completely sequenced. Southern blot analysis confirmed the presence of two copies of the TS gene in the MaHV-1 genome as expected. Computer analysis of the TS ORF showed it was 948 nucleotides in length. A putative polyadenylation signal was identified 17-22 bp inside the ORF implying a minimal or absent 3' untranslated region. The predicted polypeptide was 316 amino acid residues in length and contained the highly conserved motifs for folate binding and F-dUMP binding, typical of all TS enzymes. Interestingly, MaHV-1 TS polypeptide had highest similarity to the human TS polypeptide (81%) compared to the TS polypeptides of other herpesviruses (72-75%). Immediately upstream of the TS gene, a second ORF of 510 bp, encoding a polypeptide with 170 amino acid residues, was identified. The carboxyl domain of this MaHV-1 polypeptide shared 68% similarity to a 59 amino acid motif of human herpesvirus 1 ICP34.5, identifying it as the MaHV-1 ICP34.5 homologue. This is the first report of a herpesvirus that encodes for both TS and ICP34.5.
Keyword Genetics & Heredity
Virology
Icp34.5
Macropodid Herpesvirus
Thymidylate Synthase
Varicella-zoster Virus
Parma Wallaby Herpesvirus
Dna-sequence
Neurovirulence
Synthetase
Genome
Fibroblasts
Generation
Expression
Similarity
Q-Index Code C1
Additional Notes DOI: 10.1023/A:1015396430131

 
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