Inhibitors of COP-mediated Transport and Cholera Toxin Action Inhibit Simian Virus 40 Infection

Richards, Ayanthi A., Stang, Espen, Pepperkok, Rainer and Parton, Robert G. (2002) Inhibitors of COP-mediated Transport and Cholera Toxin Action Inhibit Simian Virus 40 Infection. Molecular Biology of The Cell, 13 5: 1750-1764. doi:10.1091/mbc.01-12-0592

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Author Richards, Ayanthi A.
Stang, Espen
Pepperkok, Rainer
Parton, Robert G.
Title Inhibitors of COP-mediated Transport and Cholera Toxin Action Inhibit Simian Virus 40 Infection
Journal name Molecular Biology of The Cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2002-05
Sub-type Article (original research)
DOI 10.1091/mbc.01-12-0592
Open Access Status File (Publisher version)
Volume 13
Issue 5
Start page 1750
End page 1764
Total pages 15
Place of publication USA
Publisher American Society for Cell Biology
Collection year 2002
Language eng
Subject C1
270103 Protein Targeting and Signal Transduction
730118 Organs, diseases and abnormal conditions not elsewhere classified
Abstract Simian virus 40 (SV40) is a nonenveloped virus that has been shown to pass from surface caveolae to the endoplasmic reticulum in an apparently novel infectious entry pathway. We now show that the initial entry step is blocked by brefeldin A and by incubation at 20degreesC. Subsequent to the entry step, the virus reaches a domain of the rough endoplasmic reticulum by an unknown pathway. This intracellular trafficking pathway is also brefeldin A sensitive. Infection is strongly inhibited by expression of GTP-restricted ADP-ribosylation factor 1 (Arf1) and Sar1 mutants and by microinjection of antibodies to betaCOP. In addition, we demonstrate a potent inhibition of SV40 infection by the dipeptide N-benzoyl-oxycarbonyl-Gly-Phe-amide, which also inhibits late events in cholera toxin action. Our results identify novel inhibitors of SV40 infection and show that SV40 requires COPI- and COPII-dependent transport steps for successful infection.
Keyword Cell Biology
Class-i Molecules
Endoplasmic-reticulum
Brefeldin-a
Golgi-apparatus
Membrane Domains
Cell-surface
Intracellular-transport
Retrograde Transport
Epithelial-cells
Beta-cop
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Created: Tue, 14 Aug 2007, 18:56:23 EST