Parenteral and mucosal delivery of a novel multi-epitope M protein-based group A streptococcal vaccine construct: investigation of immunogenicity in mice

Dunn, L. A., McMillan, D. J., Batzloff, M., Zeng, W. G., Jackson, D. C. J., Upcroft, J. A., Upcroft, P. and Olive, C. (2002) Parenteral and mucosal delivery of a novel multi-epitope M protein-based group A streptococcal vaccine construct: investigation of immunogenicity in mice. Vaccine, 20 21-22: 2635-2640. doi:10.1016/S0264-410X(02)00206-2


Author Dunn, L. A.
McMillan, D. J.
Batzloff, M.
Zeng, W. G.
Jackson, D. C. J.
Upcroft, J. A.
Upcroft, P.
Olive, C.
Title Parenteral and mucosal delivery of a novel multi-epitope M protein-based group A streptococcal vaccine construct: investigation of immunogenicity in mice
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-410X
Publication date 2002-06
Sub-type Article (original research)
DOI 10.1016/S0264-410X(02)00206-2
Volume 20
Issue 21-22
Start page 2635
End page 2640
Total pages 6
Editor R. E. Spier
Place of publication United Kingdom
Publisher Elsevier Science
Collection year 2002
Language eng
Subject C1
730213 Preventive medicine
321206 Preventive Medicine
1103 Clinical Sciences
Abstract Primary vaccine strategies against group A streptococci (GAS) have focused on the M protein-the target of opsonic antibodies important for protective immunity. We have previously reported protection of mice against GAS infection following parenteral delivery of a multi-epitope vaccine construct, referred to as a heteropolymer. This current report has assessed mucosal (intranasal (i.n.) and oral) delivery of the heteropolymer in mice with regard to the induction and specificity of mucosal and systemic antibody responses, and compared this to parenteral delivery. GAS-specific IgA responses were detected in saliva and gut upon i.n. and oral delivery of the heteropolymer co-administered with cholera toxin B subunit, respectively. High titre serum IgG responses were elicited to the heteropolymer following all routes of delivery when administered with adjuvant. Moreover, as with parenteral delivery, serum IgG antibodies were detected to the individual heteropolymer peptides following i.n. but not oral delivery. These data support the potential of the i.n. route in the mucosal delivery of a GAS vaccine. (C) 2002 Elsevier Science Ltd. All rights reserved.
Keyword Immunology
Medicine, Research & Experimental
Veterinary Sciences
Group A Streptococci
Multi-epitope
Mucosal Delivery
Group-a Streptococci
Systemic Antibody-responses
B-cell Epitopes
Cholera-toxin
Rheumatic-fever
Intranasal Immunization
Synthetic Peptides
Conserved Region
T-cell
Recombinant
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 10 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 18:54:30 EST