Comparison between human and animal isolates of Shiga toxin-producing Escherichia coli O157 from Australia

Fegan, N and Desmarchelier, P (2002) Comparison between human and animal isolates of Shiga toxin-producing Escherichia coli O157 from Australia. Epidemiology And Infection, 128 3: 357-362. doi:10.1017/S0950268801006689


Author Fegan, N
Desmarchelier, P
Title Comparison between human and animal isolates of Shiga toxin-producing Escherichia coli O157 from Australia
Journal name Epidemiology And Infection   Check publisher's open access policy
ISSN 0950-2688
Publication date 2002-01-01
Sub-type Article (original research)
DOI 10.1017/S0950268801006689
Volume 128
Issue 3
Start page 357
End page 362
Total pages 6
Editor S. Macintyre
Place of publication United Kingdom
Publisher Cambridge University Press
Collection year 2002
Language eng
Subject C1
320401 Medical Bacteriology
730113 Digestive system and disorders
Abstract There is very little human disease associated with enterohaemorrhagic Escherichia coli O157 in Australia even though these organisms are present in the animal population. A group of Australian isolates of E. coli O157:H7 and O157:H- from human and animal sources were tested for the presence of virulence markers and compared by XbaI DNA macrorestriction analysis using pulsed-field gel electrophoresis (PFGE). Each of 102 isolates tested contained the gene eae which encodes the E. coli attaching and effacing factor and all but one carried the enterohaemolysin gene, ehxA, found on the EHEC plasmid. The most common Shiga toxin gene carried was stx(2c), either alone (16%) or in combination with stx(1) (74%) or stx(2) (3%) PFGE grouped the isolates based on H serotype and some clusters were source specific. Australian E. coli O157:H7 and H- isolates from human, animal and meat sources carry all the virulence markers associated with EHEC disease in humans therefore other factors must be responsible for the low rates of human infection in Australia.
Keyword Public, Environmental & Occupational Health
Infectious Diseases
Field Gel-electrophoresis
Hemolytic-uremic Syndrome
Molecular Characterization
Hemorrhagic Colitis
Multiplex Pcr
Strains
Cattle
Sheep
Genes
Identification
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Wed, 15 Aug 2007, 04:42:35 EST