Bracken (Pteridium aquilinum)-induced DNA adducts in mouse tissues are different from the adduct induced by the activated form of the bracken carcinogen ptaquiloside

Freitas, R. N., OConnor, P. J., Prakash, A. S., Shahin, M. and Povey, A. C. (2001) Bracken (Pteridium aquilinum)-induced DNA adducts in mouse tissues are different from the adduct induced by the activated form of the bracken carcinogen ptaquiloside. Biochemical And Biophysical Research Communications, 281 2: 589-594. doi:10.1006/bbrc.2001.4388


Author Freitas, R. N.
OConnor, P. J.
Prakash, A. S.
Shahin, M.
Povey, A. C.
Title Bracken (Pteridium aquilinum)-induced DNA adducts in mouse tissues are different from the adduct induced by the activated form of the bracken carcinogen ptaquiloside
Journal name Biochemical And Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
Publication date 2001
Sub-type Article (original research)
DOI 10.1006/bbrc.2001.4388
Open Access Status
Volume 281
Issue 2
Start page 589
End page 594
Total pages 6
Place of publication USA
Publisher Academic press
Collection year 2002
Language eng
Subject C1
321299 Public Health and Health Services not elsewhere classified
730210 Environmental health
Abstract Following treatment with bracken fern (Pteridium aquilinum) extract and bracken spores a number of DNA adducts were detected by P-32-postlabeling. Three of these adducts have been described previously (Povey et al., Br. J. Cancer (1996) 74, 1342-1348) and in this study, using a slightly different protocol, four new adducts, with higher chromatographic mobility, were detected at levels ranging from 50 to 230% of those previously described, When DNA was treated in vitro with activated ptaquiloside (APT) and analysed by butanol extraction or nuclease P1 treatment, only one adduct was detected by P-32-postlabeling, This adduct was not present in the DNA from mice treated with bracken fern or spores, suggesting either that bracken contains genotoxins other than ptaquiloside or that the metabolism of ptaquiloside produces genotoxins not reflected by activated ptaquiloside. However, as the ATP-derived adduct has been detected previously in ileal DNA of bracken-fed calves, species-specific differences in the metabolism of bracken genotoxins may exist, thereby leading to differences in their biological outcomes. (C) 2001 Academic Press.
Keyword Biochemistry & Molecular Biology
Biophysics
Bracken Fern
Ptaquiloside
Dna Adducts
P-32-postlabelling
Pteridium-aquilinum
P-32-postlabeling Test
Fern
Rats
Enhancement
Sensitivity
Nucleotides
Cleavage
Cc-1065
Diet
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: National Research Centre for Environmental Toxicology Publications
 
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Created: Tue, 14 Aug 2007, 18:38:54 EST