A Mutation in the Flavin-containing Monooxygenase 3 Gene and its Effects on Catalytic Activity for N-oxidation of Trimethylamine In Vitro

Kubota, Megumi, Nakamoto, Yoko, Nakayama, Kazuo, Ujjin, Pailin, Satarug, Soisungwan, Mushiroda, Taisei, Yokoi, Tsuyoshi, Funayama, Masato and Kamataki, Tetsuya (2002) A Mutation in the Flavin-containing Monooxygenase 3 Gene and its Effects on Catalytic Activity for N-oxidation of Trimethylamine In Vitro. Drug Metabolism and Pharmacokinetics, 17 3: 207-213. doi:10.2133/dmpk.17.207


Author Kubota, Megumi
Nakamoto, Yoko
Nakayama, Kazuo
Ujjin, Pailin
Satarug, Soisungwan
Mushiroda, Taisei
Yokoi, Tsuyoshi
Funayama, Masato
Kamataki, Tetsuya
Title A Mutation in the Flavin-containing Monooxygenase 3 Gene and its Effects on Catalytic Activity for N-oxidation of Trimethylamine In Vitro
Journal name Drug Metabolism and Pharmacokinetics   Check publisher's open access policy
ISSN 1347-4367
Publication date 2002
Sub-type Article (original research)
DOI 10.2133/dmpk.17.207
Open Access Status DOI
Volume 17
Issue 3
Start page 207
End page 213
Total pages 7
Place of publication Tokyo, Japan
Publisher Japanese Society for Study of Xenobiotics
Collection year 2002
Language eng
Subject C1
321299 Public Health and Health Services not elsewhere classified
730210 Environmental health
Formatted abstract
To clarify the mutation of the flavin-containing monooxygenase (FMO) 3 gene causing fish-odor syndrome, we analyzed the FMO3 gene of a Thai subject who possibly suffered from fish-odor syndrome. A novel mutation, a single-base substitution from G to A at the position of 265 (G265A), was identified in exon 3. The mutation caused an amino acid substitution from valine to isoleucine at residue 58 (V58I). The mutated FMO3 protein with V58I exhibited the reduced trimethylamine N-oxidase activity when it was expressed in E. coli. The Vmax/Km value for the activity of the mutant-type FMO3 was about 5 times lower than that for the wild-type FMO3.
Keyword FMO3
fish-odor syndrome
trimethylamine
trimethylaminuria
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: National Research Centre for Environmental Toxicology Publications
 
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Created: Tue, 14 Aug 2007, 18:35:07 EST