In vivo DNA electrotransfer

Trezise, Anne E. O. (2002) In vivo DNA electrotransfer. DNA and Cell Biology, 21 12: 869-877. doi:10.1089/104454902762053837


Author Trezise, Anne E. O.
Title In vivo DNA electrotransfer
Journal name DNA and Cell Biology   Check publisher's open access policy
ISSN 1044-5498
Publication date 2002-06
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1089/104454902762053837
Volume 21
Issue 12
Start page 869
End page 877
Total pages 9
Place of publication New York, N.Y. U.S.A.
Publisher Mary Ann Liebert Inc. Publ
Collection year 2002
Language eng
Subject C1
270502 Neurobiology
780105 Biological sciences
Abstract The use of electrotransfer for DNA delivery to prokaryotic cells, and eukaryotic cells in vitro, has been well known and widely used for many years. However, it is only recently that electric fields have been used to enhance DNA transfer to animal cells in vivo, and this is known as DNA electrotransfer or in vivo DNA electroporation. Some of the advantages of this method of somatic cell gene transfer are that it is a simple method that can be used to transfer almost any DNA construct to animal cells and tissues in vivo; multiple constructs can be co-transfected; it is equally applicable to dividing and nondividing cells; the DNA of interest does not need to be subeloned into a specific viral transfer vector and there is no need for the production of high titre viral stocks; and, as no viral genes are expressed there is less chance of an adverse immunologic reaction to vector sequences. The ease with which efficient in vivo gene transfer can be achieved with in vivo DNA electrotransfer is now allowing genetic analysis to be applied to a number of classic animal model systems where transgenic and embryonic stem cell techniques are not well developed, but for which a wealth of detailed descriptive embryological information is available, or surgical manipulation is much more feasible. As well as exciting applications in developmental biology, in vivo DNA electrotransfer is also being used to transfer genes to skeletal muscle and drive expression of therapeutically active proteins, and to examine exogenous gene and protein function in normal adult cells situated within the complex environment of a tissue and organ system in vivo. Thus, in effect providing the in vivo equivalent of the in vitro transient transfection assay. As the widespread use of in vivo electroporation has really only just begun, it is likely that the future will hold many more applications for this technology in basic research, biotechnology and clinical research areas.
Keyword Biochemistry & Molecular Biology
Cell Biology
Genetics & Heredity
Mediated Gene-transfer
Electric-field Distribution
In-vivo
Skeletal-muscle
Subcutaneous Tumors
Plasmid Dna
Rat-liver
Electroporation
Electrochemotherapy
Cells
Q-Index Code C1

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
 
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Created: Tue, 14 Aug 2007, 18:28:00 EST