Accounting for co-extractable compounds (blank correction) in spectrophotometric measurement of extractable and total-bound proanthocyanidin in Leucaena spp

Dalzell, S. A. and Kerven, G. L. (2002) Accounting for co-extractable compounds (blank correction) in spectrophotometric measurement of extractable and total-bound proanthocyanidin in Leucaena spp. Journal of The Science of Food and Agriculture, 82 8: 860-868. doi:10.1002/jsfa.1106


Author Dalzell, S. A.
Kerven, G. L.
Title Accounting for co-extractable compounds (blank correction) in spectrophotometric measurement of extractable and total-bound proanthocyanidin in Leucaena spp
Journal name Journal of The Science of Food and Agriculture   Check publisher's open access policy
ISSN 0022-5142
Publication date 2002
Sub-type Article (original research)
DOI 10.1002/jsfa.1106
Volume 82
Issue 8
Start page 860
End page 868
Total pages 9
Place of publication Chichester, England
Publisher John Wiley & Sons on behalf of SCI
Collection year 2002
Language eng
Subject C1
300201 Plant Biochemistry and Physiology
630200 Pasture, Browse and Fodder Crops
300606 Agroforestry
Formatted abstract Methods to account for the spectral interference of co-extractable compounds (blank correction) in the spectrophotometric analysis of both extractable and bound proanthocyanidin (PA) using the proanthocyanidin (butanol/HCl) assay were evaluated. Crude extractable and bound PA sample matrices of PA-free Leucaena magnifica were used. Extractable PA blanks generated in heated 95% butanol/5% H2O reagent underestimated the optical density (absorbance) of co-extractable compounds by 24% (P < 0.01), whereas unheated 95% butanol/5% HCl blanks, incubated at room temperature, accurately measured the absorbance of the background matrix (P < 0.01). Current procedures that estimate bound PA concentrations using the proanthocyanidin assay produce intensely coloured background matrices. Recovery measurements from total-bound PA extracts spiked with 1071 and 2142 µg anthocyanidin per tube indicated that existing analytical procedures that do not account for the spectral interference of co-extractable compounds overestimated (P < 0.01) bound PA concentrations by 69 and 38% respectively. An innovative technique that generated an internal correction factor for each sample, using wavelength-scanning spectrophotometry and non-linear curve-fitting computer software, was developed. This procedure recovered 100% of added anthocyanidins from bound PA matrices.
© 2002 Society of Chemical Industry
Keyword Agriculture, Multidisciplinary
Chemistry, Applied
Food Science & Technology
Proanthocyanidin
Condensed Tannin
Extractable
Bound
Fibre-bound
Protein-bound
Insoluble
Methodology
Blank Correction
Co-extractable Compounds
Leucaena
Condensed Tannin Concentration
Dry-matter Degradation
Forage Legumes
Protein
Leaves
Browse
Fiber
Sheep
Reactivity
Phenolics
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Agriculture and Food Sciences
 
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