Axonal regeneration of retinal ganglion cells after optic nerve pre-lesions and attachment of normal or pre-degenerated peripheral nerve grafts

You, SW, Bedi, KS, Yip, HK and So, KF (2002) Axonal regeneration of retinal ganglion cells after optic nerve pre-lesions and attachment of normal or pre-degenerated peripheral nerve grafts. Visual Neuroscience, 19 5: 661-668.


Author You, SW
Bedi, KS
Yip, HK
So, KF
Title Axonal regeneration of retinal ganglion cells after optic nerve pre-lesions and attachment of normal or pre-degenerated peripheral nerve grafts
Journal name Visual Neuroscience   Check publisher's open access policy
ISSN 0952-5238
Publication date 2002
Sub-type Article (original research)
DOI 10.1017/S0952523802195113
Volume 19
Issue 5
Start page 661
End page 668
Total pages 8
Place of publication New York
Publisher Cambridge University Press
Collection year 2002
Language eng
Subject C1
270106 Cell Development (incl. Cell Division and Apoptosis)
780105 Biological sciences
Abstract Axonal regeneration of retinal ganglion cells (RGCs) into a normal or pre-degenerated peripheral nerve graft after an optic nerve pre-lesion was investigated. A pre-lesion performed 1-2 weeks before a second lesion has been shown to enhance axonal regeneration in peripheral nerves (PN) but not in optic nerves (ON) in mammals. The lack of such a beneficial pre-lesion effect may be due to the long delay (1-6 weeks) between the two lesions since RGCs and their axons degenerate rapidly 1-2 weeks following axotomy in adult rodents. The present study examined the effects of the proximal and distal ON pre-lesions with a shortened delay (0-8 days) on axonal regeneration of RGCs through a normal or pre-degenerated PN graft. The ON of adult hamsters was transected intraorbitallv at 2 mm. (proximal lesion) or intracranially at 7 mm (distal lesion) from the optic disc. The pre-lesioned ON was re-transected at 0.5 mm from the disc after 0, 1, 2, 4, or 8 days and a normal or a pre-degenerated PN graft was attached onto the ocular stump. The number of RGCs regenerating their injured axons into the PN graft was estimated by retrograde labeling with FluoroGold 4 weeks after grafting. The number of regenerating RGCs decreased significantly when the delay-time increased in animals with both the ON pre-lesions (proximal or distal) compared to control animals without an ON pre-lesion. The proximal ON pre-lesion significantly reduced the number of regenerating RGCs after a delay of 8 days in comparison with the distal lesion. However, this adverse effect can be overcome, to some degree, by a pre-degenerated PN graft applied 2, 4, or 8 days after the distal ON pre-lesion enhanced more RGCs to regenerate than the normal PN graft. Thus, in order to obtain the highest number of regenerating RGCs, a pre-degenerated PN should be grafted immediately after an ON lesion.
Keyword Retinal Ganglion Cell
Optic Nerve
Regeneration
Transplantation
Pre-lesion
Neurosciences
Ophthalmology
Rat Sciatic-nerve
Myelin-associated Glycoprotein
Conditioning Lesion
Schwann-cells
Adult-rats
Neurite Growth
Messenger-rna
Beta-tubulin
Time Course
Axotomy
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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