Quorum sensing is not required for twitching motility in Pseudomonas aeruginosa

Beatson, S. A., Whitchurch, C. B., Semmler, A. B. T. and Mattick, J. S. (2002) Quorum sensing is not required for twitching motility in Pseudomonas aeruginosa. Journal of Bacteriology, 184 13: 3598-3604. doi:10.1128/JB.184.13.3598-3604.2002

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Author Beatson, S. A.
Whitchurch, C. B.
Semmler, A. B. T.
Mattick, J. S.
Title Quorum sensing is not required for twitching motility in Pseudomonas aeruginosa
Journal name Journal of Bacteriology   Check publisher's open access policy
ISSN 0021-9193
Publication date 2002
Sub-type Article (original research)
DOI 10.1128/JB.184.13.3598-3604.2002
Open Access Status File (Publisher version)
Volume 184
Issue 13
Start page 3598
End page 3604
Total pages 7
Editor Philip Matsumura
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2002
Language eng
Abstract It has been reported that mutations in the quorum-sensing genes lasI and rhlI in Pseudomonas aeruginosa result in, among many other things, loss of twitching motility (A. Glessner, R. S. Smith, B. H. Iglewski, and J. B. Robinson, J. Bacteriol. 181:1623-1629, 1999). We constructed knockouts of lasI and rhlI and the corresponding regulatory genes lasR and rhlR and found no effect on twitching motility. However, twitching-defective variants accumulated during culturing of lasI and rhlI mutants. Further analysis showed that the stable twitching-defective variants of lasI and rhlI mutants had arisen as a consequence of secondary mutations in vfr and algR, respectively, both of which encode key regulators affecting a variety of phenotypes, including twitching motility. In addition, when grown in shaking broth culture, lasI and rhlI mutants, but not the wild-type parent, also accumulated unstable variants that lacked both twitching motility and swimming motility and appeared to be identical in phenotype to the S1 and S2 variants that were recently reported to occur at high frequencies in P. aeruginosa strains grown as a biofilm or in static broth culture (E. Deziel, Y. Comeau, and R. Villemur, J. Bacteriol. 183:1195-1204, 2001). These results indicate that mutations in one regulatory system may create distortions that select during subsequent culturing for compensatory mutations in other regulatory genes within the cellular network. This problem may have compromised some past studies of regulatory hierarchies controlled by quorum sensing and of bacterial regulatory systems in general.
Keyword Microbiology
Type-4 Fimbrial Biogenesis
Gram-negative Bacteria
Virulence Factors
Receptor Protein
Cloning Vectors
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 85 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 14 Aug 2007, 18:04:58 EST