The relationship of natural killer cell counts, perforin mRNA and CD2 expression to post-exercise natural killer cell activity in humans

Miles, M.P., Mackinnon, L.T., Grove, D.S., Williams, N.I., Bush, J.A., Marx, J.O., Kraemer, V.J. and Mastro, A.M. (2002) The relationship of natural killer cell counts, perforin mRNA and CD2 expression to post-exercise natural killer cell activity in humans. Acta Physiologica Scandinavica, 174 4: 317-325. doi:10.1046/j.1365-201x.2002.00958.x


Author Miles, M.P.
Mackinnon, L.T.
Grove, D.S.
Williams, N.I.
Bush, J.A.
Marx, J.O.
Kraemer, V.J.
Mastro, A.M.
Title The relationship of natural killer cell counts, perforin mRNA and CD2 expression to post-exercise natural killer cell activity in humans
Journal name Acta Physiologica Scandinavica   Check publisher's open access policy
ISSN 0001-6772
1365-201X
Publication date 2002
Sub-type Article (original research)
DOI 10.1046/j.1365-201x.2002.00958.x
Volume 174
Issue 4
Start page 317
End page 325
Total pages 9
Editor B. Uvnas
Place of publication Oxford, United Kingdom
Publisher Blackwell Science
Collection year 2002
Language eng
Subject C1
321401 Exercise Physiology
730102 Immune system and allergy
Abstract The aim of this study was to further investigate the mechanism of suppression of natural killer (NK) cell cytotoxic activity In peripheral blood following strenuous exercise. Blood was collected for analysis of NK cell concentration, cytotoxic activity, CD2 surface expression and perforin gene expression from runners (RUN, n = 6) and resting controls (CONTROL, n = 4) pre-exercise, 0, 1.5, 5, and 24 h following a 60-min treadmill run at 80% of VO2 peak. Natural killer cytotoxic activity, measured using a whole blood chromium release assay, fluctuated minimally in the CONTROL group and increased by 63% and decreased by 43% 0 and 1.5 h post-exercise, respectively, in the RUN group (group x time, P < 0.001). Lytic index (cytotoxic activity per cell) did not change. Perforin mRNA, measured using quantitative real-time polymerase chain reaction (ORT-PCR) decreased from pre- to post-exercise and remained decreased through 24 h, The decrease from pre- to 0 In post-exercise was seen predominately in the RUN group and was inversely correlated r = - 0.95) to pre-exercise perform mRNA. The NK cell surface expression of CD2 (lymphocyte function-associated antigen-2) was determined using fluorescent antibodies and flow cytometry, There was no change in the proportion of NK cells expressing CD2 or CD2 density, We conclude that (1) numerical redistribution accounted for most of the change in NK cytotoxic activity following a strenuous run, (2) decrease in perforin gene expression during the run was inversely related to pre-exercise levels but did not parallel changes in cytotoxic activity, and (3) CD2 surface expression was not affected by exercise.
Keyword Physiology
Adhesion
Cytotoxicity
Gene Expression
Lymphocytes
Nk Cells
Immune Function
Granzyme-b
T-cells
Activation
Indomethacin
Modulation
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Human Movement and Nutrition Sciences Publications
 
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Created: Tue, 14 Aug 2007, 18:03:08 EST