Exercise and T-lymphocyte function: a comparison of proliferation in PBMC and NK cell-depleted PBMC culture

Green, Katherine J., Rowbottom, David G. and Mackinnon, Laurel T. (2002) Exercise and T-lymphocyte function: a comparison of proliferation in PBMC and NK cell-depleted PBMC culture. Journal of Applied Physiology, 92 6: 2390-2395. doi:10.1152/japplphysiol.00926.2001


Author Green, Katherine J.
Rowbottom, David G.
Mackinnon, Laurel T.
Title Exercise and T-lymphocyte function: a comparison of proliferation in PBMC and NK cell-depleted PBMC culture
Journal name Journal of Applied Physiology   Check publisher's open access policy
ISSN 8750-7587
Publication date 2002-06
Sub-type Article (original research)
DOI 10.1152/japplphysiol.00926.2001
Volume 92
Issue 6
Start page 2390
End page 2395
Total pages 6
Editor G. C. Sieck
M. Reich
Place of publication Bethesda, MD
Publisher American Physiological Society
Collection year 2002
Language eng
Subject C1
321401 Exercise Physiology
730102 Immune system and allergy
Abstract This study utilized recently developed microbead technology to remove natural killer (NK) cells from peripheral blood mononuclear cell (PBMC) preparations to determine the effect of acute exercise on T-lymphocyte function, independent of changes in lymphocyte subpopulations. Twelve well-trained male runners completed a 60-min exercise trial at 95% ventilatory threshold and a no-exercise control trial. Six blood samples were taken at each session: before exercise, midexercise, immediately after exercise, and 30, 60, and 90 min after exercise. Isolated PBMC and NK cell-depleted PBMC were stimulated with the mitogen phytohemagglutinin. Cellular proliferation was assessed by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye uptake. In the PBMC cultures, there was a significantly lower mitogen response to phytohemagglutinin in exercise compared with the control condition immediately postexercise. There were no significant differences between the control and exercise conditions in NK cell-depleted PBMC cultures or in the responses adjusted for the percentage of CD3 cells. The present findings do not support the view that T-lymphocyte function is reduced after exercise.
Keyword Physiology
Sport Sciences
Microbeads
Cell Separation
Mitogen
Endurance Exercise
Phytohemagglutinin
Peripheral Blood Mononuclear Cells
Natural Killer Cells
Moderate-intensity Exercise
Mitogenic Response
Physical Exercise
Subpopulations
Leukocyte
Activation
Mobilization
Subsets
Assays
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Human Movement and Nutrition Sciences Publications
 
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Created: Tue, 14 Aug 2007, 18:03:06 EST