An embryo-rescue protocol for Vigna interspecific hybrids

Palmer, JL, Lawn, RJ and Adkins, SW (2002) An embryo-rescue protocol for Vigna interspecific hybrids. Australian Journal of Botany, 50 3: 331-338.


Author Palmer, JL
Lawn, RJ
Adkins, SW
Title An embryo-rescue protocol for Vigna interspecific hybrids
Journal name Australian Journal of Botany   Check publisher's open access policy
ISSN 0067-1924
Publication date 2002
Sub-type Article (original research)
DOI 10.1071/BT01086
Volume 50
Issue 3
Start page 331
End page 338
Total pages 8
Editor P.M. Attiwill
Place of publication Collingwood, Vic
Publisher CSIRO
Collection year 2002
Language eng
Subject C1
270199 Biochemistry and Cell Biology not elsewhere classified
620100 Field Crops
Abstract A successful embryo-rescue and culture protocol was developed for use with several indigenous Vigna species and mungbean cultivars grown in Australia. Germination of Vigna immature embryos and their subsequent development into plants was influenced by the time at which the embryos were isolated and by which medium additives were placed in the embryo-rescue medium. A medium containing MS basal nutrients with sucrose (88 mM), casein hydrolysate (500 mg L-1) and agar (8 g L-1) but devoid of plant-growth regulators was found to be the best for germination of immature embryos for all four Vigna species investigated. The protocol for successful germination of non-hybrid immature embryos was applied to the recovery of interspecific hybrids involving mungbean and five native Vigna species that had previously been found difficult to hybridise. Several putative hybrid plants were obtained including a confirmed interspecific cross between V. luteola (Jacq.) Benth and V. marina (Burm.) Merrill.
Keyword Plant Sciences
Hybridization
Radiata
Accessions
Mungo
Cross
Wide
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Agriculture and Food Sciences
 
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