Analysis of genetic diversity within Australian lucerne cultivars and implications for future genetic improvement

Musial, J. M., Basford, K. E. and Irwin, J. A. G. (2002) Analysis of genetic diversity within Australian lucerne cultivars and implications for future genetic improvement. Australian Journal of Agricultural Research, 53 6: 629-636.


Author Musial, J. M.
Basford, K. E.
Irwin, J. A. G.
Title Analysis of genetic diversity within Australian lucerne cultivars and implications for future genetic improvement
Journal name Australian Journal of Agricultural Research   Check publisher's open access policy
ISSN 0004-9409
Publication date 2002
Sub-type Article (original research)
DOI 10.1071/AR01178
Volume 53
Issue 6
Start page 629
End page 636
Total pages 8
Place of publication Collingwood
Publisher C S I R O Publishing
Language eng
Subject C1
270403 Plant Pathology
620000 - Plant Production and Plant Primary Products
Abstract Lucerne (Medicago sativa L.) is autotetraploid, and predominantly allogamous. This complex breeding structure maximises the genetic diversity within lucerne populations making it difficult to genetically discriminate between populations. The objective of this study was to evaluate the level of random genetic diversity within and between a selection of Australian-grown lucerne cultivars, with tetraploid M. falcata included as a possible divergent control source. This diversity was evaluated using random amplified polymorphic DNA (RAPDs). Nineteen plants from each of 10 cultivars were analysed. Using 11 RAPD primers, 96 polymorphic bands were scored as present or absent across the 190 individuals. Genetic similarity estimates (GSEs) of all pair-wise comparisons were calculated from these data. Mean GSEs within cultivars ranged from 0.43 to 0.51. Cultivar Venus (0.43) had the highest level of intra-population genetic diversity and cultivar Sequel HR (0.51) had the lowest level of intra-population genetic diversity. Mean GSEs between cultivars ranged from 0.31 to 0.49, which overlapped with values obtained for within-cultivar GSE, thus not allowing separation of the cultivars. The high level of intra- and inter-population diversity that was detected is most likely due to the breeding of synthetic cultivars using parents derived from a number of diverse sources. Cultivar-specific polymorphisms were only identified in the M. falcata source, which like M. sativa, is outcrossing and autotetraploid. From a cluster analysis and a principal components analysis, it was clear that M. falcata was distinct from the other cultivars. The results indicate that the M. falcata accession tested has not been widely used in Australian lucerne breeding programs, and offers a means of introducing new genetic diversity into the lucerne gene pool. This provides a means of maximising heterozygosity, which is essential to maximising productivity in lucerne.
Keyword Agriculture, Multidisciplinary
Medicago sativa
PCR
alfalfa
RAPD
Polymorphic Dna Markers
Tetraploid Alfalfa
Molecular Markers
Rapd
Sativa
Rflp
Populations
Germplasm
Registration
Diploids
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biological Sciences Publications
 
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