Expression analysis of delta-catenin and prostate-specific membrane antigen: Their potential as diagnostic markers for prostate cancer

Burger, M. J., Tebay, M. A., Keith, P. A., Samaratunga, H. M., Clements, J. A., Lavin, M. F. and Gardiner, R. A. (2002) Expression analysis of delta-catenin and prostate-specific membrane antigen: Their potential as diagnostic markers for prostate cancer. International Journal of Cancer, 100 2: 228-237. doi:10.1002/ijc.10468


Author Burger, M. J.
Tebay, M. A.
Keith, P. A.
Samaratunga, H. M.
Clements, J. A.
Lavin, M. F.
Gardiner, R. A.
Title Expression analysis of delta-catenin and prostate-specific membrane antigen: Their potential as diagnostic markers for prostate cancer
Journal name International Journal of Cancer   Check publisher's open access policy
ISSN 0020-7136
Publication date 2002-01-01
Sub-type Article (original research)
DOI 10.1002/ijc.10468
Volume 100
Issue 2
Start page 228
End page 237
Total pages 10
Place of publication New York
Publisher Wiley-Liss
Collection year 2002
Language eng
Subject C1
321015 Oncology and Carcinogenesis
730108 Cancer and related disorders
Abstract The current approach to prostate cancer diagnosis has major limitations including the inability of prostate-specific antigen (PSA) assays to accurately differentiate between prostate cancer and benign prostate hyperplasia (BPH) and the imprecision of transrectal ultrasound (TRUS) biopsy sampling. We have employed cDNA microarray screening to compare gene expression patterns in BPH and tumour samples to identify expression markers that may be useful in discriminating between these conditions. Screening of 3 individual cDNA arrays identified 8 genes with expression 3-fold greater in 6 tumour tissues than in 1 nontumour sample and I BPH sample. Real-time PCR was used to confirm the overexpression of these 8 genes and 12 genes selected from the literature against a panel of 17 tumours and I 1 BPH samples. Two genes, delta-catenin (delta-catenin; CTNND2) and prostate-specific membrane antigen (PSMA; FOLH1), were significantly overexpressed in prostate cancer compared to BPH. Prostate epithelial cells stained positively for S-catenin and PSMA in our prostate cancer tissues, whereas the majority of our BPH tissues were negative for both markers. Thus we have identified delta-catenin (not previously associated with prostatic adenocarcinoma) and confirmed the potential of PSMA as potential candidates for the diagnosis and management of prostate cancer. (C) 2002 Wiley-Liss. Inc.
Keyword Oncology
Prostate Cancer
Gene Profiling
Candidate Genes
Psma
Delta-catenin
Early Diagnosis
Tumor-associated Neovasculature
Normal Rectal Examinations
In-situ Hybridization
Stem-cell Antigen
Gene-expression
Alpha-catenin
Gleason Score
Beta-catenin
E-cadherin
Family
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Wed, 15 Aug 2007, 03:54:07 EST