Analysis of the sequence and expression of a second putative acetylcholinesterase cDNA from organophosphate-susceptible and organophosphate-resistant cattle ticks

Baxter, G. D. and Barker, S. C. (2002) Analysis of the sequence and expression of a second putative acetylcholinesterase cDNA from organophosphate-susceptible and organophosphate-resistant cattle ticks. Insect Biochemistry And Molecular Biology, 32 7: 815-820. doi:10.1016/S0965-1748(01)00168-0


Author Baxter, G. D.
Barker, S. C.
Title Analysis of the sequence and expression of a second putative acetylcholinesterase cDNA from organophosphate-susceptible and organophosphate-resistant cattle ticks
Journal name Insect Biochemistry And Molecular Biology   Check publisher's open access policy
ISSN 0965-1748
Publication date 2002
Sub-type Article (original research)
DOI 10.1016/S0965-1748(01)00168-0
Volume 32
Issue 7
Start page 815
End page 820
Total pages 6
Place of publication Oxford, UK
Publisher Pergamon-Elsevier
Collection year 2002
Language eng
Subject C1
300405 Animal Protection (Pests and Pathogens)
770804 Control of pests and exotic species
Abstract The cattle tick, Boophilus microplus, is a major pest of cattle in Australia, Central and South America, and parts of Africa and Asia. Control of ticks with organophosphates (OPs) and carbamates, which target acetylcholinesterases (AChE), led to evolution of resistance to these pesticides. Alleles at the locus studied here, AChE2, from OP-susceptible female ticks from Australia and Mexico differed at 46 of 1689 nucleotide positions (20 putative amino acid differences) whereas alleles from three strains of OP-resistant ticks from Australia differed with the allele from the Australian susceptible ticks at six to 13 nucleotide positions (three to six putative amino acid differences). However, the role, if any, of these polymorphisms in the OP-resistance phenotype is unknown. Certainly none of the polymorphisms correspond to sites in ACK that are involved in catalysis or binding of acetylcholine in other organisms. Both of the AChE loci of B. microplus, AChE1 and AChE2, are apparently expressed in synganglia; AChE1 is also expressed in salivary glands and ovaries, in OP-susceptible and OP-resistant ticks. This seems to contradict studies of enzyme kinetics, which indicated that only one form of AChE was present in the synganglia, the site of the action of OPs, in this species of tick. (C) 2002 Elsevier Science Ltd. All rights reserved.
Keyword Biochemistry & Molecular Biology
Entomology
Boophilus Microplus
Ixodida
Ache
Acetylcholinesterase
Insecticide
Acaricide
Nematode Caenorhabditis-elegans
Boophilus-microplus
Insensitive Acetylcholinesterase
Torpedo-californica
Molecular-forms
Culex-pipiens
Gene
Existence
Mutations
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 36 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 40 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 17:52:23 EST