Analysis of genetic diversity in Cassia brewsteri with randomly amplified DNA fingerprints (RAFS)

Cunningham, D. C., Walsh, K. B., Anderson, E. R., Harrison, D. K. and Carroll, B. J. (2002) Analysis of genetic diversity in Cassia brewsteri with randomly amplified DNA fingerprints (RAFS). Australian Systematic Botany, 15 2: 237-244.


Author Cunningham, D. C.
Walsh, K. B.
Anderson, E. R.
Harrison, D. K.
Carroll, B. J.
Title Analysis of genetic diversity in Cassia brewsteri with randomly amplified DNA fingerprints (RAFS)
Journal name Australian Systematic Botany   Check publisher's open access policy
Publication date 2002
Sub-type Article
DOI 10.1071/SB01012
Volume number 15
Issue number 2
ISSN 1030-1887
Start page 237
End page 244
Total pages 8
Place of publication Collingwood
Publisher CSIRO Publishing
Collection year 2002
Language eng
Subject C1
270401 Plant Systematics, Taxonomy and Phylogeny
620301 Native forests
Abstract Genetic diversity in Cassia brewsteri (F. Muell.) F. Muell. ex Benth. was assessed with Randomly Amplified DNA Fingerprints (RAFs). Thirty accessions of C. brewsteri collected from throughout its natural distribution were analysed with three random decamer primers, along with three accessions of C. tomentella (Benth.) Domin and a single accession of each of C. queenslandica C. T. White and C. marksiana (F. M. Bailey) Domin. The three primers yielded a reproducible amplification profile of 265 scorable polymorphic fragments for the 35 accessions. These molecular markers were used to calculate Nei and Li similarity coefficients between each pair of individuals. A matrix of dissimilarity of each pair of individuals was examined by multidimensional scaling (MDS). The analysis supports the division of C. brewsteri into two subspecies and the suggestion that intergradation of C. brewsteri and C. tomentella can occur where the distributions of these species meet.
Keyword Plant Sciences
Evolutionary Biology
Q-Index Code C1

Document type: Journal Article
Sub-type: Article
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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