Multiple shoot formation in lentil (Lens culinaris) seeds

Ye, G., McNeil, D.L., Conner, A.J. and Hill, G.D. (2002) Multiple shoot formation in lentil (Lens culinaris) seeds. New Zealand Journal of Crop And Horticultural Science, 30 1: 1-8.


Author Ye, G.
McNeil, D.L.
Conner, A.J.
Hill, G.D.
Title Multiple shoot formation in lentil (Lens culinaris) seeds
Journal name New Zealand Journal of Crop And Horticultural Science   Check publisher's open access policy
ISSN 0014-0671
Publication date 2002-03
Sub-type Article (original research)
Volume 30
Issue 1
Start page 1
End page 8
Total pages 8
Editor S. Stanislawek
Place of publication Wellington, New Zealand
Publisher The Royal Society of New Zealand
Collection year 2002
Language eng
Subject C1
300203 Plant Improvement (Selection, Breeding and Genetic Engineering)
620108 Grain legumes
Abstract A protocol based on seed culture was developed for efficient in vitro propagation of lentil (Lens culinaris Medik). Benzyladenine (BA), thidiazuron (TDZ), and kinetin all induced multiple shoot formation. In terms of the number of long shoots (>2.0 cm) produced per seed, BA and TDZ at optimum concentrations (0.2-0.4 and 0.1 mg/litre, respectively) had similar efficiency, whereas kinetin produced less shoots. Murashige and Skoog (MS) salt composition was better than that of Gamborge (B5) for shoot induction. Increasing calcium (Ca) concentration was necessary to overcome shoot-tip necrosis. For shoot elongation, fresh medium of the same composition of shoot induction medium could be used for stumps from medium with low BA (<0.8 mg/litre) or TDZ (<0.4 mg/litre). Otherwise, MS medium supplemented with 0.5 mg/litre gibberellic acid (GA(3)) or 0.05 mg/litre (alpha-naphthaleneacetic acid (NAA) was appropriate, Multiple shoots were induced from 10 inbred of four species. Best rooting was achieved using MS medium supplemented with 1.5 mg/litre NAA. Using this rooting medium more than 50% shoots from the 10 inbreds rooted. From 80 to 100% plantlets of the 10 inbreds survived after being transferred to potting mixture in glass house.
Keyword Agronomy
Horticulture
Cytokinins
Micropropagation
Seed Culture
Lentil
Lens Culinaris
Embryo Culture
Regeneration
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Tue, 14 Aug 2007, 17:50:57 EST