Alterations in Plasmodium falciparum genotypes during sequential infections suggest the presence of strain specific immunity

Eisen, D. P., Saul, A., Fryauff, D. J., Reeder, J. C. and Coppel, R. L. (2002) Alterations in Plasmodium falciparum genotypes during sequential infections suggest the presence of strain specific immunity. American Journal of Tropical Medicine And Hygiene, 67 1: 8-16.


Author Eisen, D. P.
Saul, A.
Fryauff, D. J.
Reeder, J. C.
Coppel, R. L.
Title Alterations in Plasmodium falciparum genotypes during sequential infections suggest the presence of strain specific immunity
Journal name American Journal of Tropical Medicine And Hygiene   Check publisher's open access policy
Publication date 2002
Sub-type Article
Volume number 67
Issue number 1
ISSN 0002-9637
Start page 8
End page 16
Total pages 9
Editor J. W. Kazura
M. Warren et al.
Place of publication Mclean, USA
Publisher American Society of Tropical Medicine & Hygiene
Collection year 2002
Language eng
Subject C1
321008 Haematology
730103 Blood disorders
Abstract Many of the asexual stage Plasmodium falciparum proteins that are the targets of host protective responses are markedly polymorphic. The full repertoire of diversity is not defined for any antigen. Most studies have focused on the genes encoding merozoite surface proteins 1 and 2 (MSP1, MSP2). We explored the extent of diversity of some of the less studied merozoite surface antigens and analyzed the degree of complexity of malaria field isolates by deriving nucleotide sequences of several antigens. We have determined the genotype of apical membrane antigen 1 (AMA1) in a group of 30 field samples, collected over 29 months, from individuals living in an area of intense malaria transmission in Irian Jaya, identifying 14 different alleles. AMA1 genotyping was combined with previously determined MSP2 typing. AMA1 had the greatest power in distinguishing between isolates but methodological problems, especially when mixed infections are present, suggest it is not an ideal typing target. MSP1, MSP3, and glutamate-rich protein genotypes were also determined from a smaller group of samples, and all results were combined to derive an extended antigenic haplotype. Within this subset of 10 patients, nine different genotypes could be discerned; however, five patients were all infected with the same strain. This strain was present in individuals from two separate villages and was still present 12 months later. This strain was predominant at the first time point but had disappeared at the fourth time point. This significant change in malaria genotypes could be due to strain-specific immunity developing in this population.
Keyword Public, Environmental & Occupational Health
Tropical Medicine
Merozoite Surface Protein-2
C-terminal Fragment
Apical Membrane Antigen-1
Glutamate-rich Protein
Population-structure
Humoral Response
Clinical Malaria
Allelic Variants
Irian-jaya
Ama-1
Q-Index Code C1

Document type: Journal Article
Sub-type: Article
Collections: Excellence in Research Australia (ERA) - Collection
School of Pharmacy Publications
 
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