Differential requirements for COPI coats in formation of replication complexes among three genera of Picornaviridae

Gazina, Elean V., Mackenzie, Jason M., Gorrell, Rebecca J. and Anderson, David A. (2002) Differential requirements for COPI coats in formation of replication complexes among three genera of Picornaviridae. Journal of Virology, 76 21: 11113-11122. doi:10.1128/JVI.76.21.11113-11122.2002

Author Gazina, Elean V.
Mackenzie, Jason M.
Gorrell, Rebecca J.
Anderson, David A.
Title Differential requirements for COPI coats in formation of replication complexes among three genera of Picornaviridae
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
Publication date 2002-11
Year available 2002
Sub-type Article (original research)
DOI 10.1128/JVI.76.21.11113-11122.2002
Open Access Status DOI
Volume 76
Issue 21
Start page 11113
End page 11122
Total pages 10
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2002
Language eng
Subject C1
270303 Virology
730101 Infectious diseases
730204 Child health
Abstract Picornavirus RNA replication requires the formation of replication complexes (RCs). consisting of virus-induced vesicles associated with viral nonstructural proteins and RNA. Brefeldin A (BFA) has been shown to strongly inhibit RNA replication of poliovirus but not of encephalomyocarditis virus (EMCV). Here, we demonstrate that the replication of parechovirus 1 (ParV1) is partly resistant to BFA, whereas echovirus 11 (EV11) replication is strongly inhibited. Since BFA inhibits COPI-dependent steps in endoplasmic reticulum (ER)-Golgi transport, we tested a hypothesis that different picornaviruses may have differential requirements for COPI in the formation of their RCs. Using immunofluorescence and cryo-immunoelectron microscopy we examined the association of a COPI component, beta-COP, with the RCs of EMCV, ParV1, and EV11 EMCV RCs did not contain beta-COP. In contrast, beta-COP appeared to be specifically distributed to the RCs of EV11 In ParV1-infected cells beta-COP was largely dispersed throughout the cytoplasm, with some being present in the RCs. These results suggest that there are differences in the involvement of COPI in the formation of the RCs of various picornaviruses, corresponding to their differential sensitivity to BFA. EMCV RCs are likely to be formed immediately after vesicle budding from the ER, prior to COPI association with membranes. ParV1 RCs are formed from COPI-containing membranes but COPI is unlikely to be directly involved in their formation, whereas formation of EV11 RCs appears to be dependent on COPI association with membranes.
Keyword Picornaviruses
Repliction Complexes
Membrane Origins
Brefeldin And Replication
Peripheral Membrane-protein
Flavivirus-infected Cells
To-golgi Transport
Intermediate Compartment
Poliovirus Infection
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
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Created: Tue, 14 Aug 2007, 17:29:17 EST