Fate of swallowed interleukin 8 and TNF alpha and effect on the wistar rat gut

Briars, G.L., Greer, R., Cleghorn, G. J. and Shepherd, R. W. (2002) Fate of swallowed interleukin 8 and TNF alpha and effect on the wistar rat gut. Journal of Cystic Fibrosis, 1 2: 94-98. doi:10.1016/S1569-1993(02)00035-8

Author Briars, G.L.
Greer, R.
Cleghorn, G. J.
Shepherd, R. W.
Title Fate of swallowed interleukin 8 and TNF alpha and effect on the wistar rat gut
Formatted title
Fate of swallowed interleukin 8 and TNFα, and effect on the Wistar rat gut
Journal name Journal of Cystic Fibrosis   Check publisher's open access policy
ISSN 1569-1993
Publication date 2002
DOI 10.1016/S1569-1993(02)00035-8
Volume 1
Issue 2
Start page 94
End page 98
Total pages 5
Editor H. G. M. Heijerman
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2002
Language eng
Subject C1
321019 Paediatrics
730107 Inherited diseases (incl. gene therapy)
Abstract To evaluate the passage of cytokines through the gastrointestinal tract, we investigated the digestion of interleukin-8 (IL-8) and tumour necrosis factor α (TNFα), in vitro and in vivo, and their propensity to induce intestinal inflammation. We serially immuno-assayed IL-8 and TNFα solutions co-incubated with each of three pancreatin preparations at pH 4.5 and pH 8. We gavaged IL-8, TNFα and marker into 15 Wistar rats, and measured their faecal cytokine concentrations by ELISA and histologically examined their guts. IL-8 immunoreactivity was extinguished by all pancreatin preparations after 1 h of incubation at 37 °C. TNFα concentration progressively fell from 1 to 4 h with all enzyme preparations. Buffer control samples maintained their cytokine concentrations throughout incubation. No IL-8 or TNFα was detected in any rat faecal pellets. There was no significant proinflammatory effect of the gavaged cytokines on rat intestine. IL-8 and TNFα in aqueous solution could well be fully digested in the CF gut when transit time is normal and exogenous enzymes are provided, although cytokines swallowed in viscous sputum may be protected from such digestion. Copyright © 2011 Elsevier B.V. All rights reserved
Keyword Interleukin-8
Tumor necrosis factor α (TNFα)
Pancreatic enzymes
Wistar rats
Q-Index Code C1

Version Filter Type
Citation counts: Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 17:06:01 EST