Replication of bovine papillomavirus type 1 (BPV-1) DNA in Saccharomyces cerevisiae following infection with BPV-1 virions

Zhao, Kong-Nan and Frazer, Ian H. (2002) Replication of bovine papillomavirus type 1 (BPV-1) DNA in Saccharomyces cerevisiae following infection with BPV-1 virions. Journal of Virology, 76 7: 3359-3364. doi:10.1128/JVI.76.7.3359-3364.2002


Author Zhao, Kong-Nan
Frazer, Ian H.
Title Replication of bovine papillomavirus type 1 (BPV-1) DNA in Saccharomyces cerevisiae following infection with BPV-1 virions
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2002-04
Year available 2002
Sub-type Article (original research)
DOI 10.1128/JVI.76.7.3359-3364.2002
Open Access Status DOI
Volume 76
Issue 7
Start page 3359
End page 3364
Total pages 6
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2002
Language eng
Subject C1
320402 Medical Virology
730101 Infectious diseases
Abstract Saccharomyces cerevisiae protoplasts exposed to bovine papillomavirus type 1 (BPV-1) virions demonstrated uptake of virions on electron microscopy. S. cerevisiae cells looked larger after exposure to BPV-1 virions, and cell wall regeneration was delayed. Southern blot hybridization of Hirt DNA from cells exposed to BPV-1 virions demonstrated BPV-1 DNA, which could be detected over 80 days of culture and at least 13 rounds of division. Two-dimensional gel analysis of Hirt DNA showed replicative intermediates, confirming that the BPV-1 genome was replicating within S. cerevisiae. Nicked circle, linear, and supercoiled BPV-1 DNA species were observed in Hirt DNA preparations from S. cerevisiae cells infected for over 50 days, and restriction digestion showed fragments hybridizing to BPV-1 in accord with the predicted restriction map for circular BPV-1 episomes. These data suggest that BPV-1 can infect S. cerevisiae and that BPV-1 episomes can replicate in the infected S. cerevisiae cells.
Keyword Virology
Open Reading Frame
Capsid Protein
Raft Cultures
Integrin
Yeast
Expression
Differentiation
Gene
Identification
Keratinocytes
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
UQ Diamantina Institute Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 22 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 14 Aug 2007, 17:02:52 EST