Chronic hypoxia induced down-regulation of angiotensinogen expression in rat epididymis

Leung, PS, Fung, ML and Sernia, C (2001) Chronic hypoxia induced down-regulation of angiotensinogen expression in rat epididymis. Regulatory Peptides, 96 3: 143-149. doi:10.1016/S0167-0115(00)00169-5

Author Leung, PS
Fung, ML
Sernia, C
Title Chronic hypoxia induced down-regulation of angiotensinogen expression in rat epididymis
Journal name Regulatory Peptides   Check publisher's open access policy
ISSN 0167-0115
Publication date 2001
Sub-type Article (original research)
DOI 10.1016/S0167-0115(00)00169-5
Volume 96
Issue 3
Start page 143
End page 149
Total pages 7
Editor MI Phillips
WE Schmidt
Place of publication Netherlands
Publisher Elsevier Science
Collection year 2001
Language eng
Subject C1
Abstract The presence of an intrinsic renin-angiotensin system (RAS) in the rat epididymis has been previously established by showing the expression of several key RAS components, and in particular angiotensinogen, the indispensable element for the intracellular generation of angiotensin II. In this study, the possible involvement of this local epididymal RAS in the testicular effects of chronic hypoxia was investigated. Semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR), Western blotting and by in situ hybridization histochemistry of the rat epididymis were used to show changes in localization and expression of angiotensinogen. Results from RT-PCR analysis demonstrated that chronic hypoxia caused a marked decrease (60%) in the expression of angiotensinogen mRNA, when compared with that in the normoxic epididymis. Western blot analysis demonstrated a less decrease (35%) in the expression of angiotensinogen protein. In situ hybridization histochemistry showed that the reduced angiotensinogen mRNA in chronic hypoxia was specifically localized to the epididymal epithelium from the cauda, corpus and caput regions of the epididymis; a distribution similar to that of normoxic rats. It was concluded that chronic hypoxia decreases the transcriptional and translational expression of angiotensinogen, and thus local formation of angiotensin II, in the rat epididymis. (C) 2001 Elsevier Science B.V. All rights reserved.
Keyword Endocrinology & Metabolism
Renin-angiotensin System
Chronic Hypoxia
Endothelial Growth-factor
Chronic Hypobaric Hypoxia
Ii Receptors
Hypertensive Rats
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 20 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 16:58:23 EST