Identifying the origin of single corneal cells by DNA fingerprinting - Part I - Implications for corneal limbal allografting

Henderson, T. R., Findlay, I., Matthews, P. L. and Noble, B. A. (2001) Identifying the origin of single corneal cells by DNA fingerprinting - Part I - Implications for corneal limbal allografting. Cornea, 20 4: 400-403. doi:10.1097/00003226-200105000-00013


Author Henderson, T. R.
Findlay, I.
Matthews, P. L.
Noble, B. A.
Title Identifying the origin of single corneal cells by DNA fingerprinting - Part I - Implications for corneal limbal allografting
Journal name Cornea   Check publisher's open access policy
ISSN 0277-3740
Publication date 2001
Sub-type Article (original research)
DOI 10.1097/00003226-200105000-00013
Volume 20
Issue 4
Start page 400
End page 403
Total pages 4
Place of publication Philadelphia, USA
Publisher Lippincott williams and Wilkins
Collection year 2001
Language eng
Subject C1
270800 Biotechnology
780108 Behavioural and cognitive sciences
1103 Clinical Sciences
1113 Ophthalmology and Optometry
Abstract Purpose. To demonstrate that the combination of impression cytology and single cell DNA fingerprinting represents a powerful tool that is suitable for detecting transplanted cells after corneal limbal allografting. Methods, Fifty single cells were obtained by corneal impression cytology from 12 patients undergoing cataract surgery. Individual cells were isolated from samples by micromanipulation. Polymerase chain reaction and short tandem repeat profiling was used to obtain forensic standard DNA fingerprints from single cells. Blood samples taken at the time of impression cytology provided control fingerprints. Results, informative DNA fingerprints were obtained from all corneal samples and 66% (33 of 50 cells) of isolated single cells, Of all fingerprints obtained, most (91%, 30 of 33 fingerprints) corneal fingerprints matched corresponding blond sample fingerprints. At least one corneal fingerprint matched the corresponding blood sample fingerprint in 83% (10 of 12 patients) of the patients in the study, Conclusions. This extremely specific single cell DNA fingerprinting system permits accurate identification of individual corneal epithelial cells, allowing very reliable determination of their origin, which will enable host and donor cells to be distinguished from each other after keratolimbal allografting procedures. even if the host and donor are the same sex or siblings. These DNA fingerprinting methods allow assessment of quality and quantity of donor cell survival, as well as survival time. The extreme sensitivity and accuracy of the technique means that should contamination occur, it would be identified, thus ensuring meaningful results.
Keyword Ophthalmology
Dna Fingerprinting
Impression Cytology
Corneal Stem Cells
Keratolimbal Allograft
Polymerase Chain-reaction
Ocular Surface
Transplantation
Survival
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Tue, 14 Aug 2007, 16:57:03 EST