The effects of pregnancy on myelin basic protein-induced experimental autoimmune encephalomyelitis in Lewis rats: Suppression of clinical disease, modulation of cytokine expression in the spinal cord inflammatory infiltrate and suppression of lymphocyte p

Harness, J. and McCombe, P. A. (2001) The effects of pregnancy on myelin basic protein-induced experimental autoimmune encephalomyelitis in Lewis rats: Suppression of clinical disease, modulation of cytokine expression in the spinal cord inflammatory infiltrate and suppression of lymphocyte p. American Journal of Reproductive Immunology, 46 6: 405-412. doi:10.1111/%28ISSN%291600-0897


Author Harness, J.
McCombe, P. A.
Title The effects of pregnancy on myelin basic protein-induced experimental autoimmune encephalomyelitis in Lewis rats: Suppression of clinical disease, modulation of cytokine expression in the spinal cord inflammatory infiltrate and suppression of lymphocyte p
Journal name American Journal of Reproductive Immunology   Check publisher's open access policy
ISSN 1046-7408
0271-7352
Publication date 2001
Sub-type Article (original research)
DOI 10.1111/%28ISSN%291600-0897
Volume 46
Issue 6
Start page 405
End page 412
Total pages 8
Editor Norbert Gleicher
Place of publication Copenhagen, Denmark
Publisher Blackwell Munksgaard
Collection year 2001
Language eng
Subject C1
320207 Autoimmunity
730102 Immune system and allergy
1107 Immunology
Abstract Problem: The present study was performed to explore the effects of pregnancy on experimental autoimmune encephalomyelitis (EAE) induced in Lewis rats by inoculation with myelin basic protein (MBP) (MBP-EAE). Method of study: MBP-EAE was induced in pregnant and non-pregnant rats and severity of disease evaluated. Serum from pregnant and non-pregnant rats was used in standard lymphocyte proliferation assays. Real-time polymerase chain reaction (PCR) was used to investigate the expression of cytokine mRNA in the inflammatory cells obtained from the spinal cord of rats on day 15 after inoculation. Results: Pregnant rats developed less severe disease than non-pregnant rats. Serum from pregnant rats suppressed the proliferation of T lymphocytes in response to MBP. There was significantly increased expression of IL-4. IL-10 and TNF-alpha mRNA in the spinal cord infiltrate of pregnant rats. Conclusion: Circulating humoral factors and alteration in cytokine production by inflammatory cells may contribute to the suppression of EAE in pregnant rats.
Keyword Immunology
Reproductive Biology
Cytokines
Eae
Encephalomyelitis
Immunosuppression
Multiple Sclerosis
Polymerase Chain Reaction
Pregnancy
Experimental Allergic Encephalomyelitis
Central-nervous-system
Messenger-rna Expression
Multiple-sclerosis
Interferon-gamma
Ifn-gamma
T-cells
Molecular-cloning
Factor-beta
Mice
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Tue, 14 Aug 2007, 16:56:19 EST