Phenotypic characterization of five dendritic cell subsets in human tonsils

Summers, Kelly L., Hock, Barry D., McKenzie, Judith L. and Hart, Derek N. J. (2001) Phenotypic characterization of five dendritic cell subsets in human tonsils. American Journal of Pathology, 159 1: 285-295. doi:10.1016/S0002-9440(10)61694-X

Author Summers, Kelly L.
Hock, Barry D.
McKenzie, Judith L.
Hart, Derek N. J.
Title Phenotypic characterization of five dendritic cell subsets in human tonsils
Journal name American Journal of Pathology   Check publisher's open access policy
ISSN 0002-9440
Publication date 2001-07
Sub-type Article (original research)
DOI 10.1016/S0002-9440(10)61694-X
Volume 159
Issue 1
Start page 285
End page 295
Total pages 11
Place of publication Birmingham, AL, U.S.A.
Publisher American Society for Investigative Pathology
Collection year 2001
Language eng
Subject C1
320202 Cellular Immunology
730102 Immune system and allergy
Abstract Heterogeneous expression of several antigens on the three currently defined tonsil dendritic cell (DC) subsets encouraged us to re-examine tonsil DCs using a new method that minimized DC differentiation and activation during their preparation. Three-color flow cytometry and dual-color immunohistology was used in conjunction with an extensive panel of antibodies to relevant DC-related antigens to analyze lin(-) HLA-DR+ tonsil DCs. Here we identify, quantify, and locate five tonsil DC subsets based on their relative expression of the HLA-DR, CD11c, CD13, and CD123 antigens. In situ localization identified four of these DC subsets as distinct interdigitating DC populations. These included three new interdigitating DC subsets defined as HLA-DRhi CD11c(+) DCs, HLA-DRmod CD11c+ CD13(+) DCs, and HLA-DRmod CD11c(-) CD123(-) DCs, as well as the plasmacytoid DCs (HLA-DRmod CD11c- CD123(+)). These subsets differed in their expression of DC-associated differentiation/activation antigens and co-stimulator molecules including CD83, CMRF-44, CMRF-56, 2-7, CD86, and 4-1BB ligand. The fifth HLA-DRmod CD11c(+) DC subset was identified as germinal center DCs, but contrary to previous reports they are redefined as lacking the CD13 antigen. The definition and extensive phenotypic analysis of these five DC subsets In human tonsil extends our understanding of the complexity of DC biology.
Keyword Pathology
Naive B-cells
Allostimulatory Cells
Induced Apoptosis
Langerhans Cells
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
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Created: Tue, 14 Aug 2007, 16:51:23 EST