Re-evaluation of the cytokinin receptor role of the Arabidopsis gene GCR1

Humphrey, Tania Vivienne and Botella, José Ramón (2001) Re-evaluation of the cytokinin receptor role of the Arabidopsis gene GCR1. Journal of Plant Physiology, 158 5: 645-653. doi:10.1078/0176-1617-00316

Author Humphrey, Tania Vivienne
Botella, José Ramón
Title Re-evaluation of the cytokinin receptor role of the Arabidopsis gene GCR1
Journal name Journal of Plant Physiology   Check publisher's open access policy
ISSN 0176-1617
Publication date 2001
Sub-type Article (original research)
DOI 10.1078/0176-1617-00316
Volume 158
Issue 5
Start page 645
End page 653
Total pages 9
Place of publication Jena, Germany
Publisher Urban & Fischer Verlag
Collection year 2001
Language eng
Subject C1
270201 Gene Expression
620299 Horticultural crops not elsewhere classified
Abstract GCR1 has been tentatively identified in Arabidopsis thaliana as the first plant G-protein coupled receptor (GPCR) (Josefsson and Rask 1997) implicated in the cytokinin sensory pathway (Plakidou-Dymock et al. 1998). A protein fusion of GCR1 and green fluorescent protein has been expressed in Arabidopsis and shown GCR1 to be located on the plasma membrane. Studies of plants with altered GCR1 expression have led us to question GCR1's involvement in cytokinin signaling. Transgenic Arabidopsis plants containing sense and antisense constructs for GCR1 have been produced and over- and under-expression confirmed. The analysis of 12 antisense and 17 sense lines has failed to reveal the previously reported Dainty phenotype or altered cytokinin sensitivity. We have used the Gauntlet approach to test the plants' response to various plant hormones although this has not yet identified a mutant phenotype. The yeast-two hybrid system has been used and so far there is no evidence to suggest GCR1 interacts with heterotrimeric G proteins. Before GCR1 can be identified as genuine G-protein coupled receptor, the identification of a ligand and a proof of association with heterotrimeric G-proteins should be obtained.
Keyword Plant Sciences
Arabidopsis Thaliana
Signal Transduction
Green Fluorescent Protein
Coupled Receptor
Beta(2)-adrenergic Receptor
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
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Created: Tue, 14 Aug 2007, 16:50:13 EST