Drug susceptibility testing of anaerobic protozoa

Upcroft, JA and Upcroft, P (2001) Drug susceptibility testing of anaerobic protozoa. Antimicrobial Agents And Chemotherapy, 45 6: 1810-1814. doi:10.1128/AAC.45.6.1810-1814.2001

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Author Upcroft, JA
Upcroft, P
Title Drug susceptibility testing of anaerobic protozoa
Journal name Antimicrobial Agents And Chemotherapy   Check publisher's open access policy
ISSN 0066-4804
Publication date 2001
Sub-type Article (original research)
DOI 10.1128/AAC.45.6.1810-1814.2001
Open Access Status File (Publisher version)
Volume 45
Issue 6
Start page 1810
End page 1814
Total pages 5
Editor G.M. Eliopoulos
Place of publication Washimgton DC
Publisher American Society of Microbiology
Collection year 2001
Language eng
Subject C1
320405 Medical Parasitology
730101 Infectious diseases
Abstract A simple technique for routine, reproducible global surveillance of the drug susceptibility status of the anaerobic protozoa Trichomonas, Entamoeba, and Giardia is described, Data collected using this technique can be readily compared among different laboratories and with previously reported data. The technique employs a commercially available sachet and bag system to generate a low-oxygen environment and log, drug dilutions in microtiter plates, which can be monitored without aerobic exposure, to assay drug-resistant laboratory lines and clinically resistant isolates. MICs (after 2 days) of 3.2 and 25 muM indicated metronidazole-sensitive and highly clinically resistant isolates of T. vaginalis in anaerobic assays, respectively. The aerobic MICs were 25 and > 200 muM. MICs (1 day) of 12.5 to 25 muM were found for axenic lines of E. histolytica, and MICs for G. duodenalis (3 days) ranged from 6.3 muM for metronidazole-sensitive isolates to 50 muM for laboratory metronidazole-resistant lines. This technique should encourage more extensive monitoring of drug resistance in these organisms.
Keyword Microbiology
Pharmacology & Pharmacy
Trichomonas-vaginalis
Entamoeba-histolytica
Metronidazole Resistance
Giardia-intestinalis
Superoxide-dismutase
Hiv Transmission
In-vitro
Invitro
Albendazole
Duodenalis
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Tue, 14 Aug 2007, 16:49:14 EST