Transcripts of the MHM region on the chicken Z chromosome accumulate as non-coding RNA in the nucleus of female cells adjacent to the DMRT1 locus

Teranishi, M., Shimada, Y., Hori, T., Nakabayashi, O., Kikuchi, T., MacLeod, T., Pym, R. A., Sheldon, B., Solovei, I., Macgregor, H. and Shigeki, M (2001) Transcripts of the MHM region on the chicken Z chromosome accumulate as non-coding RNA in the nucleus of female cells adjacent to the DMRT1 locus. Chromosome Research, 9 2: 147-165. doi:10.1023/A:1009235120741


Author Teranishi, M.
Shimada, Y.
Hori, T.
Nakabayashi, O.
Kikuchi, T.
MacLeod, T.
Pym, R. A.
Sheldon, B.
Solovei, I.
Macgregor, H.
Shigeki, M
Title Transcripts of the MHM region on the chicken Z chromosome accumulate as non-coding RNA in the nucleus of female cells adjacent to the DMRT1 locus
Journal name Chromosome Research   Check publisher's open access policy
ISSN 0967-3849
Publication date 2001
Sub-type Article (original research)
DOI 10.1023/A:1009235120741
Volume 9
Issue 2
Start page 147
End page 165
Total pages 19
Place of publication Netherlands
Publisher Kluwer Academic
Collection year 2001
Language eng
Subject C1
300599 Veterinary Sciences not elsewhere classified
630106 Poultry
0707 Veterinary Sciences
Abstract The male hypermethylated (MHM) region, located near the middle of the short arm of the Z chromosome of chickens, consists of approximately 210 tandem repeats of a BamHI 2.2-kb sequence unit. Cytosines of the CpG dinucleotides of this region are extensively methylated on the two Z chromosomes in the male but much less methylated on the single Z chromosome in the female. The state of methylation of the MHM region is established after fertilization by about the 1-day embryonic stage. The MHM region is transcribed only in the female from the particular strand into heterogeneous, high molecular-mass, non-coding RNA, which is accumulated at the site of transcription, adjacent to the DMRT1 locus, in the nucleus. The transcriptional silence of the MHM region in the male is most likely caused by the CpG methylation, since treatment of the male embryonic fibroblasts with 5-azacytidine results in hypo-methylation and active transcription of this region. In ZZW triploid chickens, MHM regions are hypomethylated and transcribed on the two Z chromosomes, whereas MHM regions are hypermethylated and transcriptionally inactive on the three Z chromosomes in ZZZ triploid chickens, suggesting a possible role of the W chromosome on the state of the MHM region.
Keyword Biochemistry & Molecular Biology
Genetics & Heredity
Cpg Methylation
Dmrt1 Gene
Gallus Gallus Domesticus
Mhm Region
Nuclear Non-coding Rna
Triploid Chickens
W Chromosome
Z Chromosome
Sex-determining Region
X-inactivation Center
W-chromosome
Gallus-domesticus
Conserved Synteny
Gene
Expression
Embryos
Mouse
Human-chromosome-9
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 89 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 89 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 16:39:15 EST