A test of the ash-free dry weight technique on the developmental stages of Patiriella spp. (Echinodermata : Asteroidea)

Moreno, Guillermo, Selvakumaraswamy, Paulina, Byrne, Maria and Hoegh-Guldberg, Ove (2001) A test of the ash-free dry weight technique on the developmental stages of Patiriella spp. (Echinodermata : Asteroidea). Limnology And Oceanography, 46 5: 1214-1220. doi:10.4319/lo.2001.46.5.1214

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Author Moreno, Guillermo
Selvakumaraswamy, Paulina
Byrne, Maria
Hoegh-Guldberg, Ove
Title A test of the ash-free dry weight technique on the developmental stages of Patiriella spp. (Echinodermata : Asteroidea)
Journal name Limnology And Oceanography   Check publisher's open access policy
ISSN 0024-3590
Publication date 2001-07-12
Sub-type Article (original research)
DOI 10.4319/lo.2001.46.5.1214
Open Access Status File (Publisher version)
Volume 46
Issue 5
Start page 1214
End page 1220
Total pages 7
Place of publication Waco, TX, United States
Publisher American Society of Limnology and Oceanography
Collection year 2001
Language eng
Subject C1
270603 Animal Physiology - Systems
770402 Land and water management
Abstract Determination of the ash-free dry weight (AFDW) of marine specimens requires samples to be rinsed, soaked, and centrifuged. Problems associated with this technique were examined with the developmental stages of seastar species (Patiriella) with different modes of development. The influence of three rinsing solutions (ammonium formate [AF], filtered seawater [FSW], and reverse osmosis water [RO]) was assessed. The hypothesis that the AFDW technique is a measure of organic material was addressed by drying inorganic salts. Developmental stages of Patiriella calcar rinsed in FSW were twice as heavy as those rinsed in RO or AE indicating that samples should be rinsed in RO or AF before weighing. Soaking treatments had a significant effect on the AFDW of samples of P. calcar (planktonic developer), indicating that the rinsing period should be brief. Zygotes of Patiriella re gularis (planktonic developer) were significantly heavier than ova or gastrulae, regardless of treatment. In contrast, there were no significant differences in the AFDW of any stages or treatments of Patiriella exigua (benthic developer). This may be due to the presence of a modified fertilization envelope, which protects these benthic embryos. Inorganic salts with water of crystallization and FSW lost 20-75% and 14% of their dry weight, respectively, after ashing. We propose that salt ions may retain water, which does not evaporate during drying but is lost during ashing, resulting in the overestimation of sample AFDW. If a similar process occurs in the developmental stages of marine invertebrates, changes in the intracellular ionic composition through development may result in inaccurate estimates of biomass.
Keyword Limnology
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Marine Studies Publications
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 14 Aug 2007, 16:32:40 EST