Differentiation of peanut seedborne potyviruses and curcumoviruses by RT-PCR

Dietzgen, R. G., Callaghan, B., Higgins, C. M., Birch, R. G., Chen, K. and Xu, Z. (2001) Differentiation of peanut seedborne potyviruses and curcumoviruses by RT-PCR. Plant Disease, 85 9: 989-992. doi:10.1094/PDIS.2001.85.9.989

Author Dietzgen, R. G.
Callaghan, B.
Higgins, C. M.
Birch, R. G.
Chen, K.
Xu, Z.
Title Differentiation of peanut seedborne potyviruses and curcumoviruses by RT-PCR
Journal name Plant Disease   Check publisher's open access policy
ISSN 0191-2917
Publication date 2001
Sub-type Article (original research)
DOI 10.1094/PDIS.2001.85.9.989
Volume 85
Issue 9
Start page 989
End page 992
Total pages 4
Editor A.R. Biggs
Place of publication St Paul, USA
Publisher The American Phytopathological Society
Collection year 2001
Language eng
Subject C1
270400 Botany
620100 Field Crops
Abstract Seedborne peanut viruses pose important constraints to peanut production and safe movement of germ plasm. They also pose a risk of accidental introduction into previously disease-free regions. We have developed reverse transcription-polymerase chain reaction (RT-PCR) assays based on identical cycling parameters which identified peanut stripe, Peanut mottle, Peanut stunt, and Cucumber mosaic viruses through production of specific DNA fragments of 234 bp, 327 bp, 390 bp, and 133 bp, respectively. Assay sensitivity in the picogram range was achieved. The two potyviruses and two cucumoviruses could be differentiated using duplex RT-PCR assays. These assays should be useful for testing peanut leaves or seeds for virus identification in epidemiological studies, seed testing or in post-entry quarantine.
Keyword Plant Sciences
Duplex Rt-pcr
Virus Diagnosis
Polymerase Chain-reaction
Cucumber Mosaic-virus
Stripe Potyvirus
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
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Created: Tue, 14 Aug 2007, 16:31:56 EST