A multifunctional polyketide-peptide synthetase essential for albicidin biosynthesis in Xanthomonas albilineans

Huang, Guozhong, Zhang, Lianhui and Birch, Robert G. (2001) A multifunctional polyketide-peptide synthetase essential for albicidin biosynthesis in Xanthomonas albilineans. Microbiology, 147 3: 631-642.


Author Huang, Guozhong
Zhang, Lianhui
Birch, Robert G.
Title A multifunctional polyketide-peptide synthetase essential for albicidin biosynthesis in Xanthomonas albilineans
Journal name Microbiology   Check publisher's open access policy
ISSN 1350-0872
Publication date 2001-03
Sub-type Article (original research)
Volume 147
Issue 3
Start page 631
End page 642
Total pages 12
Editor M. V. Ivanov
Place of publication Reading, England
Publisher Society for General Microbiology
Collection year 2001
Language eng
Subject C1
270300 Microbiology
620100 Field Crops
Abstract Albicidins, a family of potent antibiotics and phytotoxins produced by the sugarcane leaf scald pathogen Xanthomonas albilineans, inhibit DNA replication in bacteria and plastids. A gene located by Tn5-tagging was confirmed by complementation to participate in albicidin biosynthesis. The gene (xabB) encodes a large protein (predicted Mr 525695), with a modular architecture indicative of a multifunctional polyketide synthase (PKS) linked to a non-ribosomal peptide synthetase (NRPS). At 4801 amino acids in length, XabB is the largest reported PKS–NRPS. Twelve catalytic domains in this multifunctional enzyme are arranged in the order N terminus–acyl-CoA ligase (AL)–acyl carrier protein (ACP)–ß-ketoacyl synthase (KS)–ß-ketoacyl reductase (KR)–ACP–ACP–KS–peptidyl carrier protein (PCP)–condensation (C)–adenylation–PCP–C. The modular architecture of XabB indicates likely steps in albicidin biosynthesis and approaches to enhance antibiotic yield. The novel pattern of domains, in comparison with known PKS–NRPS enzymes for antibiotic production, also contributes to the knowledge base for rational design of enzymes producing novel antibiotics.
Keyword leaf scald phytotoxin
nonribosomal peptide synthetase
polyketide synthase
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Tue, 14 Aug 2007, 16:31:48 EST