Rapid transplacental infection with bovine pestivirus following intranasal inoculation of ewes in early pregnancy

Swasdipan, S., Bielefeldt-Ohmann, H., Phillips, N., Kirkland, P. D. and McGowan, M. R. (2001) Rapid transplacental infection with bovine pestivirus following intranasal inoculation of ewes in early pregnancy. Veterinary Pathology, 38 3: 275-280. doi:10.1354/vp.38-3-275


Author Swasdipan, S.
Bielefeldt-Ohmann, H.
Phillips, N.
Kirkland, P. D.
McGowan, M. R.
Title Rapid transplacental infection with bovine pestivirus following intranasal inoculation of ewes in early pregnancy
Journal name Veterinary Pathology   Check publisher's open access policy
ISSN 0300-9858
Publication date 2001
Sub-type Article (original research)
DOI 10.1354/vp.38-3-275
Open Access Status Not Open Access
Volume 38
Issue 3
Start page 275
End page 280
Total pages 6
Editor Donna F. Kusewitt
Place of publication US
Publisher Basel:Karger
Collection year 2001
Language eng
Subject C1
300402 Animal Reproduction
630100 Livestock
Abstract Despite the importance of congenital viral infections in both veterinary and human medicine, only limited experimental work has been carried out to elucidate the mechanisms involved in transplacental virus infections. To further an understanding of fetal infection with pestiviruses, the distribution of bovine pestivirus in the uterine and fetal tissues of ewes in early pregnancy, following a natural route of infection, was investigated. On the 18th day of pregnancy, nine ewes were inoculated by the intranasal route with 1 X 10(5) 50% tissue culture infective doses of an Australian isolate of noncytopathic bovine pestivirus (bovine viral diarrhea virus genotype 1). All ewes were ovariohysterectomized at approximately 100 hours postinfection. Samples from the reproductive tract and conceptus were examined histologically and tested for bovine pestivirus by nested reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry and for interferon-tau mRNA expression by nonnested RT-PCR. Although no histopathologic changes were observed in the maternal or fetal tissues, virus was detected in the reproductive tract of all nine ewes and in all of the conceptuses examined. Al; the time of surgery, only two of the nine ewes were demonstrably viremic. This study demonstrates that bovine pestivirus can spread from a natural site of infection to the ovine fetus within 4 days in the absence of maternal immunity and despite the presence of interferon expression in the reproductive tract.
Keyword Bovine Pestivirus
Intranasal Inoculation
Pathology
Veterinary Sciences
Immunohistochemistry
Interferons
Polymerase Chain Reaction
Sheep
Trans-placental Infection
Viral Diarrhea Virus
Fetal
Expression
Disease
Tissues
Cells
Fetus
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Tue, 14 Aug 2007, 16:19:59 EST