Assembly and maturation of the flavivirus Kunjin virus appear to occur in the rough endoplasmic reticulum and along the secretory pathway, respectively

MacKenzie, Jason M. and Westaway, Edwin G. (2001) Assembly and maturation of the flavivirus Kunjin virus appear to occur in the rough endoplasmic reticulum and along the secretory pathway, respectively. Journal of Virology, 75 22: 10787-10799. doi:10.1128/JVI.75.22.10787-10799.2001


Author MacKenzie, Jason M.
Westaway, Edwin G.
Title Assembly and maturation of the flavivirus Kunjin virus appear to occur in the rough endoplasmic reticulum and along the secretory pathway, respectively
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2001-11
Year available 2001
Sub-type Article (original research)
DOI 10.1128/JVI.75.22.10787-10799.2001
Open Access Status DOI
Volume 75
Issue 22
Start page 10787
End page 10799
Total pages 13
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2001
Language eng
Subject C1
270303 Virology
730101 Infectious diseases
Abstract The intracellular assembly site for flaviviruses in currently not known but is presumed to be located within the lumen of the rough endoplasmic reticulum (RER), Building on previous studies involving immunofluorescence (IF) and cryoimmunoelectron microscopy of Kunjin virus (KUN)-infected cells, we sought to identify the steps involved in the assembly and maturation of KUN. Thus, using antibodies directed against envelope protein E in IF analysis, we found the accumulation of E within regions coincident with the RER and endosomal compartments. Immunogold labeling of cryosections of infected cells indicated that E and minor envelope protein prM were localized to reticulum membranes continuous with KUN-induced convoluted membranes (CM) or paracrystalline arrays (PC) and that sometimes the RER contained immunogold-labeled virus particles. Both proteins were also observed to be labeled in membranes at the periphery of the induced CIM or PC structures, but the latter were very seldom labeled internally. Utilizing drugs that inhibit protein and/or membrane traffic throughout the cell, we found that the secretion of KUN particles late in infection was significantly affected in the presence of brefeldin A and that the infectivity of secreted particles was severely affected in the presence of monensin and N-nonyl-deoxynojirimycin. Nocodazole did not appear to affect maturation, suggesting that microtubules play no role in assembly or maturation processes. Subsequently, we showed that the exit of intact virions from the RER involves the transport of individual virions within individual vesicles en route to the Golgi apparatus. The results suggest that the assembly of virions occurs within the lumen of the RER and that subsequent maturation occurs via the secretory pathway.
Keyword Virology
Borne Encephalitis-virus
Alpha-glucosidase Inhibitors
Vesicular Stomatitis-virus
Cultured Mosquito Cells
Amino-acid Sequences
Brefeldin-a
Membrane-glycoproteins
Envelope Glycoprotein
Golgi-apparatus
Infected Cells
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Clinical Medical Virology Centre Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 201 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 208 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 16:05:08 EST