Phylogenetic analysis of evolutionary relationships of the planctomycete division of the domain bacteria based on amino acid sequences of elongation factor Tu

Jenkins, C. and Fuerst, J. A. (2001) Phylogenetic analysis of evolutionary relationships of the planctomycete division of the domain bacteria based on amino acid sequences of elongation factor Tu. Journal of Molecular Evolution, 52 5: 405-418.


Author Jenkins, C.
Fuerst, J. A.
Title Phylogenetic analysis of evolutionary relationships of the planctomycete division of the domain bacteria based on amino acid sequences of elongation factor Tu
Journal name Journal of Molecular Evolution   Check publisher's open access policy
ISSN 0022-2844
Publication date 2001
Sub-type Article (original research)
Volume 52
Issue 5
Start page 405
End page 418
Total pages 14
Place of publication Berlin
Publisher Springer
Collection year 2001
Language eng
Subject C1
270301 Bacteriology
780105 Biological sciences
0601 Biochemistry and Cell Biology
0603 Evolutionary Biology
0604 Genetics
Abstract Sequences from the tuf gene coding for the elongation factor EF-Tu were amplified and sequenced from the genomic DNA of Pirellula marina and Isosphaera pallida, two species of bacteria within the order Planctomycetales. A near-complete (1140-bp) sequence was obtained from Pi. marina and a partial (759-bp) sequence was obtained for I. pallida. Alignment of the deduced Pi. marina EF-Tu amino acid sequence against reference sequences demonstrated the presence of a unique Il-amino acid sequence motif not present in any other division of the domain Bacteria. Pi. marina shared the highest percentage amino acid sequence identity with I. pallida but showed only a low percentage identity with other members of the domain Bacteria. This is consistent with the concept of the planctomycetes as a unique division of the Bacteria. Neither primary sequence comparison of EF-Tu nor phylogenetic analysis supports any close relationship between planctomycetes and the chlamydiae, which has previously been postulated on the basis of 16S rRNA. Phylogenetic analysis of aligned EF-Tu amino acid sequences performed using distance, maximum-parsimony, and maximum likelihood approaches yielded contradictory results with respect to the position of planctomycetes relative to other bacteria, It is hypothesized that long-branch attraction effects due to unequal evolutionary rates and mutational saturation effects may account for some of the contradictions.
Keyword Biochemistry & Molecular Biology
Evolutionary Biology
Genetics & Heredity
Planctomycetes
Planctomycetales
Elongation Factor
Ef-tu
Domain Bacteria
Long-branch Attraction
Deep-branching Divisions
Ribosomal-rna Sequences
Budding Bacteria
Nucleotide-sequence
Thermotoga-maritima
Signature Sequences
Aquifex-pyrophilus
Protein Phylogeny
Universal Tree
Rrn Operons
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Tue, 14 Aug 2007, 16:03:43 EST