Schistosoma japonicum cathepsin D aspartic protease cleaves human IgG and other serum components

Verity, C. K., Loukas, A., McManus, D. P. and Brindley, P. J. (2001) Schistosoma japonicum cathepsin D aspartic protease cleaves human IgG and other serum components. Parasitology, 122 4: 415-421. doi:10.1017/S0031182001007521


Author Verity, C. K.
Loukas, A.
McManus, D. P.
Brindley, P. J.
Title Schistosoma japonicum cathepsin D aspartic protease cleaves human IgG and other serum components
Journal name Parasitology   Check publisher's open access policy
ISSN 0031-1820
Publication date 2001
Sub-type Article (original research)
DOI 10.1017/S0031182001007521
Volume 122
Issue 4
Start page 415
End page 421
Total pages 7
Place of publication New York
Publisher Cambridge University Press
Collection year 2001
Language eng
Subject C1
320405 Medical Parasitology
730118 Organs, diseases and abnormal conditions not elsewhere classified
Abstract Recombinant cathepsin D aspartic protease of Schistosoma japonicum cleaved human IgG in vitro in a time and dose-dependent manner. Optimal cleavage was seen at pH 3.6-4.5; modest cleavage remained at pH 5.0, and no cleavage was detected above pH 5.0. Amino terminal sequencing of the major cleavage fragments of human IgG identified a Fab fragment from the VH1 domain, and 2 cleavage sites in the CH2 domain below the hinge region. The P1 and P1' residues at the 2 CH2 cleavage sites were Phe254-Leu255 and Leu325-Thr326, indicating a preference by the schistosome protease for bulky hydrophobic residues flanking the scissile bond. No cleavage of the immunoglobulin light chain was detected. In addition, the recombinant schistosome protease indiscriminately degraded the human serum proteins complement C3 and serum albumin into numerous small fragments. These results demonstrate specific cleavage of human IgG by the recombinant schistosome aspartic protease, and highlight the broad range digestive specificity of the enzyme which may play a role in the degradation of host serum proteins ingested as part of the schistosome bloodmeal.
Keyword Parasitology
Cathepsin D
Schistosoma Japonicum
Igg
Proteolysis
Fasciola-hepatica
Procathepsin-d
Proteinase
Mansoni
Degradation
Hemoglobin
Activation
Fragments
Pepstatin
Database
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Tue, 14 Aug 2007, 15:52:44 EST