A potential role for the analytical ultracentrifuge in the experimental measurement of protein valence

Winzor, D. J., Carrington, L. E. and Harding, S. E. (2001) A potential role for the analytical ultracentrifuge in the experimental measurement of protein valence. Analytical Biochemistry, 299 2: 235-240.


Author Winzor, D. J.
Carrington, L. E.
Harding, S. E.
Title A potential role for the analytical ultracentrifuge in the experimental measurement of protein valence
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 0003-2697
Publication date 2001
Sub-type Article (original research)
DOI 10.1006/abio.2001.5408
Volume 299
Issue 2
Start page 235
End page 240
Total pages 6
Place of publication California
Publisher Academic Press
Collection year 2001
Language eng
Subject C1
270100 Biochemistry and Cell Biology
780105 Biological sciences
Abstract A method is described whereby sedimentation velocity is combined with equilibrium dialysis to determine the net charge (valence) of a protein by using chromate as an indicator ion for assessing the extent of the Donnan redistribution of small ions. The procedure has been used in experiments on bovine serum albumin under slightly alkaline conditions (pH 8.0, I 0.05) to illustrate its application to a system in which the indicator ion and protein both bear net negative charge and on lysozyme under slightly acidic conditions (pH 5.0, I 0.10) to illustrate the situation where chromate is a counterion. (C) 2001 Elsevier Science.
Keyword Biochemical Research Methods
Biochemistry & Molecular Biology
Chemistry, Analytical
Sedimentation Equilibrium Experiments
Thermodynamic Nonideality
Electrophoresis
Charge
Interference
Coefficient
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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Created: Tue, 14 Aug 2007, 15:18:21 EST