Crystallization and preliminary X-ray diffraction studies of FHA domains of Dun1 and Rad53 protein kinases

Blanchard, H., Fontes, R. M, Hammet, A., Pike, B. L., Teh, T., Gleichmann, T., Gooley, P. R., Kobe, B. and Heierhorst, J. (2001) Crystallization and preliminary X-ray diffraction studies of FHA domains of Dun1 and Rad53 protein kinases. Acta Crystallographica Section D: Biological Crystallography, 57 3: 459-461. doi:10.1107/S0907444901002116

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Author Blanchard, H.
Fontes, R. M
Hammet, A.
Pike, B. L.
Teh, T.
Gleichmann, T.
Gooley, P. R.
Kobe, B.
Heierhorst, J.
Title Crystallization and preliminary X-ray diffraction studies of FHA domains of Dun1 and Rad53 protein kinases
Journal name Acta Crystallographica Section D: Biological Crystallography   Check publisher's open access policy
ISSN 0907-4449
Publication date 2001
Sub-type Article (original research)
DOI 10.1107/S0907444901002116
Open Access Status File (Publisher version)
Volume 57
Issue 3
Start page 459
End page 461
Total pages 3
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell
Collection year 2001
Language eng
Subject C1
270100 Biochemistry and Cell Biology
780105 Biological sciences
Abstract Forkhead-associated (FHA) domains are modular protein–protein interaction domains of ~130 amino acids present in numerous signalling proteins. FHA-domain-dependent protein interactions are regulated by phosphorylation of target proteins and FHA domains may be multifunctional phosphopeptide-recognition modules. FHA domains of the budding yeast cell-cycle checkpoint protein kinases Dun1p and Rad53p have been crystallized. Crystals of the Dun1-FHA domain exhibit the symmetry of the space group P6122 or P6522, with unit-cell parameters a = b = 127.3, c = 386.3 Å; diffraction data have been collected to 3.1 Å resolution on a synchrotron source. Crystals of the N-terminal FHA domain (FHA1) of Rad53p diffract to 4.0 Å resolution on a laboratory X-ray source and have Laue-group symmetry 4/mmm, with unit-cell parameters a = b = 61.7, c = 104.3 Å.
Keyword Biochemical Research Methods
Biochemistry & Molecular Biology
Biophysics
Crystallography
Dna-damage
Checkpoint
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Tue, 14 Aug 2007, 15:17:26 EST