The expression of plasminogen activator system in a rat model of periodontal wound healing

Xiao, Y., Li, H. K., Bunn, C. and Bartold, P. M. (2001) The expression of plasminogen activator system in a rat model of periodontal wound healing. Journal of Periodontology, 72 7: 849-857. doi:10.1902/jop.2001.72.7.849

Author Xiao, Y.
Li, H. K.
Bunn, C.
Bartold, P. M.
Title The expression of plasminogen activator system in a rat model of periodontal wound healing
Journal name Journal of Periodontology   Check publisher's open access policy
ISSN 0022-3492
Publication date 2001-07
Sub-type Article (original research)
DOI 10.1902/jop.2001.72.7.849
Volume 72
Issue 7
Start page 849
End page 857
Total pages 9
Editor Robert Genco
Place of publication Chicago
Publisher American Society of Periodontology
Collection year 2001
Language eng
Subject C1
320899 Dentistry not elsewhere classified
730112 Oro-dental and disorders
1105 Dentistry
Abstract Background: The plasminogen activator system has been proposed to play a role in proteolytic degradation of extracellular matrices in tissue remodeling, including wound healing. The aim of this study was to elucidate the presence of components of the plasminogen activator system during different stages of periodontal wound healing. Methods: Periodontal wounds were created around the molars of adult rats and healing was followed for 28 days. Immunohistochemical analyses of the healing tissues and an analysis of the periodontal wound healing fluid by ELISA were carried out for the detection of tissue-type plasminogen activator (t-PA), urokinase-type plasminogen activator (u-PA), and 2 plasminogen activator inhibitors (PAI-1 and PAI-2). Results: During the early stages (days 1 to 3) of periodontal wound healing, PAI-1 and PAI-2 were found to be closely associated with the deposition of a fibrin clot in the gingival sulcus. These components were strongly associated with the infiltrating inflammatory cells around the fibrin clot. During days 3 to 7, u-PA, PAI-1, and PAI-2 were associated with cells (particularly monocytes/macrophages, fibroblasts, and endothelial cells) in the newly formed granulation tissue. During days 7 to 14, a new attachment apparatus was formed during which PAI-1, PAI-2, and u-PA were localized in both periodontal ligament fibroblasts (PDL) and epithelial cells at sites where these cells were attaching to the root surface. In the periodontal wound healing fluid, the concentration for t-PA increased and peaked during the first week. PAI-2 had a similar expression to t-PA, but at a lower level over the entire wound-healing period. Conclusions: These findings indicate that the plasminogen activator system is involved in the entire process of periodontal wound healing, in particular with the formation of fibrin matrix on the root surface and its replacement by granulation tissue, as well as the subsequent formation of the attachment of soft tissue to the root surface during the later stages of wound repair.
Keyword Dentistry, Oral Surgery & Medicine
Animal Studies
Plasminogen Activator
Tissue Plasminogen Activator
Extracellular Matrix
Wound Healing
Periodontal Diseases/therapy
Inhibitor Type-2
Skin Wounds
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 14 Aug 2007, 15:13:04 EST