Control of the cystic fibrosis transmembrane conductance regulator by alpha G(i) and RGS proteins

Schreiber, R, Kindle, P, Benzing, T, Walz, G and Kunzelmann, K (2001) Control of the cystic fibrosis transmembrane conductance regulator by alpha G(i) and RGS proteins. Biochemical And Biophysical Research Communications, 281 4: 917-923. doi:10.1006/bbrc.2001.4455


Author Schreiber, R
Kindle, P
Benzing, T
Walz, G
Kunzelmann, K
Title Control of the cystic fibrosis transmembrane conductance regulator by alpha G(i) and RGS proteins
Journal name Biochemical And Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
Publication date 2001-01-01
Sub-type Article (original research)
DOI 10.1006/bbrc.2001.4455
Volume 281
Issue 4
Start page 917
End page 923
Total pages 7
Place of publication United States
Publisher Academic Press Inc
Collection year 2001
Language eng
Subject C1
270104 Membrane Biology
730110 Respiratory system and diseases (incl. asthma)
Abstract The cystic fibrosis transmembrane conductance regulator (CFTR) has been shown previously to be regulated by inhibitory G proteins. In the present study, we demonstrate inhibition of CFTR by alphaG(i2) and alphaG(i1), but not alphaG(0), in Xenopus oocytes. We further examined whether regulators of G protein signaling (RGS) proteins interfere with alphaG(i)-dependent inhibition of CFTR. Activation of CFTR by IBMX and forskolin was attenuated in the presence of alphaG(i2), indicating inhibition of CFTR by alphaG(i2) in Xenopus oocytes. Coexpression of the proteins RGS3 and RGS7 together with CFTR and alphaG(i2) partially recovered activation by IBMX/forskolin. 14-3-3, a protein that is known to interfere with RGS proteins, counteracted the effects of RGS3. These data demonstrate the regulation of CFTR by alphaG(i) in Xenopus oocytes. Because RGS proteins interfere with the G protein-dependent regulation of CFTR, this may offer new potential pathways for pharmacological intervention in cystic fibrosis. (C) 2001 Academic Press.
Keyword Biochemistry & Molecular Biology
Biophysics
Cftr
Regulators Of G Protein Signaling
Rgs3
Rgs7
14-3-3
Alpha G(i2)
Alpha G(i1) Cystic Fibrosis
Xenopus Oocytes
Epithelial-cells
Cl Channel
Trafficking
Activation
Modulation
G(q-alpha)
Beta
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Wed, 15 Aug 2007, 01:11:56 EST